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Neisseria Meningitidis Antigen Orf1 Antikörper

Antigen

Neisseria Meningitidis Antigen Orf1

Klonalität Polyklonal
Wirt

Kaninchen

Applikation
Western Blot (WB)
Produktnummer ABIN125757
Menge 0.1 mg  (1 mg/ml)
Preis 230,00 €   Zzgl. Versandkosten €40,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 3 bis 5 Werktagen

Produktbeschreibung

Weitere Bezeichnung Neisseria meningitidis antigen Orf1 / FrpD
Immunogen Slices containing purified FrpD250-6xHis-tagged protein were excised from 10%SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used toimmunize a rabbit.
Format Purified
Beschreibung Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localizationand [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with atype II signal peptide for export across the cytoplasmic membrane and is, upon processingto a lipoprotein, sorted to the outer bacterial membrane.
Spezifität The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outermembrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strainsrepresentative of all serogroups and does not exhibit any similarity to known sequences ofother organisms.

Anwendungen

Applikationshinweise Western Blotting - Recommended dilution: 0.2 - 1 µg/ml. Application note: Reducing conditions.
Konzentration 1 mg/ml
Reinheit > 95% (by SDS-PAGE)
Reinigung Purified from rabbit serum by protein-A afinity chromatography.
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Lagerung Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
Beschränkungen Nur für Forschungszwecke einsetzbar