Orf1 (N. Meningitidis) Antikörper

Produktnummer ABIN125757

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-Orf1 (N. Meningitidis) Antikörper (ABIN125757) detektiert spezifisch Orf1 (N. Meningitidis) in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Neisseria meningitidis.

Kurzübersicht für Orf1 (N. Meningitidis) Antikörper (ABIN125757)

Target: Orf1 (N. Meningitidis)

Reaktivität: Neisseria meningitidis

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-Orf1 (N. meningitidis) Purified

Kreuzreaktivität (Details): Neisseria meningitidis

Produktmerkmale: The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.

Anwendungsinformationen

Applikationshinweise: Western blotting: Recommended dilution 0.5-1 μg/mL, reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen

Prochazkova, Osicka, Linhartova, Halada, Sulc, Sebo: "The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC." in: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3251-8, (2005) (PubMed).

Antigendetails

Target: Orf1 (N. Meningitidis)

Hintergrund: Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.,FrpD

Gen-ID: 35