CD5 Molecule (CD5) Antikörper

Antigen: CD5 Molecule (CD5)

Synonyme: T1, LEU1, Ly-1, Ly-A, Ly-12, Lyt-1, MGC114334, CD5, MGC137790

Klonalität: Monoklonal (CRIS1)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN125749

Produktbeschreibung

Weitere Bezeichnung: CD5

Immunogen: stimulated human leukocytes

Format: Purified

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: CRIS1

Beschreibung: CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chaintransmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most ofthymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is atype I membrane glycoprotein whose extracellular region contains three scavenger receptorcysteine-rich (SRCR) domains.The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsiccatalytic activity. CD5 modulates signaling through the antigen-specific receptor complex(TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosinephosphorylation of intracellular proteins and DAG production. Preliminary evidence showsprotein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dualreceptor, giving either stimulatory or inhibitory signals depending both on the cell type anddevelopment stage. In thymocytes and B1a cells seems to provide inhibitory signals, inperipheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is thephenotypic marker of a B cell subpopulation involved in the production of autoreactiveantibodies.Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferativedisorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in someautoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss ofCD5 expression in the expanded CD8+ human T cells.

Spezifität: The antibody CRIS1 reacts with the cell surface glycoprotein CD5, a 67kDa single-chaintransmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes andB lymphocytes subset (B-1a lymphocytes).HLDA I, WS Code T 29HLDA III, WS Code T 530

Anwendungen

Applikationshinweise: Flow Cytometry: Recommended dilution: purified: 1 µg/ml. Positive control: Peripheral Blood Lymphocytes (PBL)Jurkat human leukemia T-cell lineHPB human leukemia T-cell lineMOLT-4 human leukemia T-cell line. - Recommended dilution: 1-2 µg/ml. Positive control: Jurkat human leukemia T-cell lineHPB human leukemia T-cell line.

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, Adaptive Immunität, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Flow Cytometry analysis of human Peripheral Blood Lymphocytes (PBL) stained with CRIS1 antibody (dilution of purified antibody 1 ug/ml), Fig. 1a Histogram - gated on lymphocytes, overlay with isotypic control (mouse IgG2a), Fig. 1b Dot plot.