HIV Protease Antikörper

Antigen: HIV Protease

Klonalität: Monoklonal (1696)

Wirt: Maus

Applikation: Western Blot (WB), Funktionale Studien (Func), ELISA

Produktnummer: ABIN125709

Produktbeschreibung

Immunogen: Bacterially expressed full-length HIV-1 protease

Format: Azide Free

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 1696

Beschreibung: The HIV protease (PR) hydrolyzes polyproteins of HIV virus into functional protein productsthat are essential for its assembly and subsequent activity. This maturation process occursas the virion buds from the host cell.HIV protease inhibitors are used in the treatment of patients with AIDS and were consideredthe first breakthrough in over a decade of AIDS research. HIV protease inhibitors can lowerthe viral load carried by AIDS patents.

Spezifität: The antibody 1696 recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2),an enzyme that hydrolyzes polyproteins of HIV viruses into functional proteins.The antibody 1696 does not react with the precursor.

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 0.5 µg/ml. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withreducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Functional Application: The antibody 1696 strongly inhibits the enzyme activity of HIV-1 and HIV-2 proteases.

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by protein-A affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Virologie, Proteasen

Beschränkungen: Nur für Forschungszwecke einsetzbar

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