P-Selectin Antikörper

Produktnummer ABIN120298

Dieser Maus Monoklonal Antikörper detektiert spezifisch P-Selectin in FACS, IP und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Schaf und Ziege.

Kurzübersicht für P-Selectin Antikörper (ABIN120298)

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reaktivität: Human, Ratte, Schaf, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser P-Selectin Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: Psel-KO-2-12

Produktdetails anti-P-Selectin Antikörper

Spezifität: SM2262P recognises the CD62P cell surface antigen, a 140 kDa glycoprotein also known as P-selectin. Clone Psel.KO.2.12 is reported to inhibit P-Selectin-dependent adhesion between HL60 cells and P-Selectin transfected COS cells (1). We recommend the use of SM2262LE for this purpose.

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: CD62P transfected 300.19 cells. Remarks: Spleen cells from immunised CD62P knock-out mice (Strain C57/B6) were fused with cells of the NS-1 myeloma cell line.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 106 cells in 100 μL. Immunohistology on Frozen Sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Andere Bezeichnung: CD62P / P-Selectin

Hintergrund: CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.Synonyms: CD62 antigen-like family member P, GMP-140, GMRP, GRMP, Granule membrane protein 140, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, PADGEM, SELP

Gen-ID: 6403

NCBI Accession: NP_002996

UniProt: P16109