MHC Class II I-Ab Antikörper (Biotin)

Antigen: MHC Class II I-Ab

Klonalität: Monoklonal (25-5-16S)

Wirt: Maus

Reaktivität: Maus

Konjugat: Biotin

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN118318

Produktbeschreibung

Isotyp: IgM

Klon: 25-5-16S

Spezifität: This antibody specifically reacts with the I-Ab encoded MHC Class II alloantigen expressed on mouse strains BXSB/Mp, C57BL/ 6, C57BL/10, LP/J, and 129. Tissue Distribution by Flow Cytometry Analysis: Representative Histogram Mouse Strain: C57BL/6 Cell Concentration : 1x10e6 cells per test Antibody Concentration Used: 0.5 ug/10e6 cells Isotypic Control: Biotin Mouse IgM.

Anwendungen

Applikationshinweise: Flow Cytometry (see protocol below). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.1 mg/ml

Buffer: PBS, 0.09% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.

Lagerung: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.

Forschungsgebiet: Haupthistokompatibilitätskomplex (MHC), Immunologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Mouse anti MHC Class II I-Ab 25.5.16S

Publikationen

Publikationen: Klein, Figueroa, David: "H-2 haplotypes, genes and antigens: second listing. II. The H-2 complex." in: Immunogenetics, Vol. 17, Issue 6, pp. 553-96, 1983 (PubMed).

Ozato, Sachs: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, 1981 (PubMed).