Integrin alpha X (ITGAX) Antikörper (FITC)

Antigen: Integrin alpha X (ITGAX)

Synonyme: CD11C, SLEB6, BR, GPIa, CD49B, VLA-2, VLAA2, VL3A, CD49C, GAPB3, MSK18, VCA-2, VLA3a, GAP-B3, FLJ34631, FLJ34704, IA4, CD49D, MGC90518, FNRA, CD49e, VLA5A, ITGAX, RGD1561123, Cr4, N418, Cd11c, AI449405, CD11c

Klonalität: Monoklonal (PS-2)

Wirt: Ratte

Reaktivität: Human, Maus

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN118225

Produktbeschreibung

Weitere Bezeichnung: CD49d / ITGA4

Swiss-Prot: P13612

Isotyp: IgG2b

Klon: PS-2

Beschreibung: Integrin a 4 (also called CD49d) is a ~150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin a 4 forms heterodimers with integrins b 1 and b 7. Integrin a 4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin a 4 promotes cell migration and inhibits cell spreading and contractility. Integrin a 4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin a 4 function has become an important target for drug discovery. Phosphorylation of the serine 988 residue on the integrin a 4 cytoplasmic tail inhibits its interaction with the focal adhesion complex-associated protein paxillin, and modulates its effects on cell motility and spreading. Alternate Names: ITGA4, Integrin alpha-IV, VLA-4, Integrin alpha-4, CD49 antigen-like family member D.

Spezifität: This antibody recognises murine alpha 4 integrin (CD49d). Clone PS/2 has also been reported to block the binding of CD49d to its ligands. We recommend the use of ABIN118226 for this purpose.

Synonyme: CD11C, SLEB6, BR, GPIa, CD49B, VLA-2, VLAA2, VL3A, CD49C, GAPB3, MSK18, VCA-2, VLA3a, GAP-B3, FLJ34631, FLJ34704, IA4, CD49D, MGC90518, FNRA, CD49e, VLA5A, ITGAX, RGD1561123, Cr4, N418, Cd11c, AI449405, CD11c

Anwendungen

Applikationshinweise: Flow Cytometry: Use 10 ul of 1/5-1/10 diluted antibody to label 10e6 cells in 100 ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.1 mg/ml

Buffer: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Shelf Life: one year from despatch.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen , Zell-/Gewebemarker, Extrazelluläre Matrix, Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Miyake, Medina, Ishihara et al.: "A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture." in: The Journal of cell biology, Vol. 114, Issue 3, pp. 557-65, 1991 (PubMed).

Miyake, Weissman, Greenberger et al.: "Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis." in: The Journal of experimental medicine, Vol. 173, Issue 3, pp. 599-607, 1991 (PubMed).