LIM Domain Binding 1 Protein Antikörper (AA 361-373)

Produktnummer ABIN118013

Der Kaninchen Polyklonal anti-LIM Domain Binding 1 Protein Antikörper wird verwendet zum Nachweis von LIM Domain Binding 1 Protein in Proben von Human, Maus, Huhn und Xenopus laevis. Er wurde validiert für EIA.

Kurzübersicht für LIM Domain Binding 1 Protein Antikörper (AA 361-373) (ABIN118013)

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Reaktivität: Human, Maus, Huhn, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIM Domain Binding 1 Protein Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA)

Produktdetails anti-LIM Domain Binding 1 Protein Antikörper

Bindungsspezifität: AA 361-373

Spezifität: This affinity purified antibody is directed against the mouse LDB1protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, chimpanzee, dog, frog, chicken and rat based on 100 % homology for the immunogen sequence. Cross reactivity with LDB1 homologues from other sources has not been determined.

Aufreinigung: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 361-373 of mouse LDB1 protein.

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA, western blot and CHIP. Specific conditions for reactivity should be optimized by the end user. Expect a bandapproximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriatecell lysate or extract. This antibody has been used in a ChIP assay using murineerythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1gene, a putative LDB1 binding region as suggested by Orkin et al. Anti-LDB1 was usedsuccessfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1enhancer element in DMSO-induced murine erythroleukemia cells. We suggest using 20 g for 10e8 cells for ChIP. ELISA: 1: 2,000-1: 8,000. Western Blot: 1: 500-1: 3,000. Immunofluorescence microscopy.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C.

Details zu LIM Domain Binding 1 Protein

Target: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Andere Bezeichnung: LDB1

Hintergrund: LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.Synonyms: CLIM-2, CLIM2, Carboxyl-terminal LIM domain-binding protein 2, LDB1, LIM domain-binding factor CLIM2, LIM domain-binding protein 1, Nuclear LIM interactor, hLdb1

Gen-ID: 16825

NCBI Accession: NP_001106879

UniProt: P70662

Pathways: Stem Cell Maintenance, Chromatin Binding