Invertase Antikörper

Antigen: Invertase

Klonalität: Polyklonal

Wirt: Ziege

Applikation: Enzyme Immunoassay (EIA), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN117966

Produktbeschreibung

Produktmerkmale: Synonyms: Beta-fructofuranosidase, Saccharase, INV1

Gen-ID: 2896726

Swiss-Prot: Q9Y746

Immunogen: Invertase [Candida]

Format: Serum

Beschreibung: Invertase hydrolyses the terminal non reducing beta D fructofuranoside residues in beta Dfructofuranosides. It is the enzyme that bees use to convert nectar into honey. Industrialand confectionary applications use yeast invertase to split sucrose into fructose andglucose and also to improve the shelf life of food products.

Spezifität: This product was prepared from monospecific antiserum. Assay by immunoelectrophoresisresulted in a single precipitin arc against purified and partially purified Invertase[Candida]. Cross reactivity against Invertase from other sources is unknown.

Anwendungen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayedagainst 1.0 ug of Invertase [Candida] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 10,000 to 1: 65,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 90.0 mg/ml (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01%(w/v) Sodium Azide as preservative.Reconstitution: Restore with 2.0 ml of deionized water (or equivalent).

Lagerung: Store vial at 2-8°C prior to restoration. Restore with 2. 0 ml of deionized water (orequivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior toimmediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Enzyme, Metabolismus

Beschränkungen: Nur für Forschungszwecke einsetzbar