Acetylcholinesterase Antikörper
Kurzübersicht für Acetylcholinesterase Antikörper (ABIN116884)
Target
Alle Acetylcholinesterase (AChE) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Spezifität
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes]. Cross reactivity against Acetyl Cholinesterase from other sources is unknown.
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Aufreinigung
- Prepared from monospecific antiserum by a delipidation and defibrination.
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Immunogen
- Acetyl Cholinesterase [Bovine Erythrocytes].
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Applikationshinweise
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Diutions: This product has been assayed against 1.0 g of AcetylCholinesterase [Bovine Erythrocytes] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 20,000 to 1: 100,000 of the reconstitution concentration issuggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Rekonstitution
- Restore with 1.0 mL of deionized water (or equivalent).
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Konzentration
- 90.0 mg/mL (by Refractometry)
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Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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Lagerung
- 4 °C/-20 °C
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Informationen zur Lagerung
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Acetylcholinesterase (AChE)
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Andere Bezeichnung
- Acetylcholinesterase
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Hintergrund
- Synonyms: AChE, Acetylcholine Esterase, Acetylcholine-Esterase
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Gen-ID
- 540446
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NCBI Accession
- NP_001069688
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UniProt
- P23795
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Pathways
- Skeletal Muscle Fiber Development
Target
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