Pancreatic Islet Lysate Antikörper

Produktnummer ABIN116793

Der Hund Polyklonal anti-Pancreatic Islet Lysate Antikörper (ABIN116793) detektiert spezifisch Pancreatic Islet Lysate in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Schwein.

Kurzübersicht für Pancreatic Islet Lysate Antikörper (ABIN116793)

Target: Pancreatic Islet Lysate

Reaktivität: Schwein

Wirt: Hund

Klonalität: Polyklonal

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails

Aufreinigung: Multi-step process.

Immunogen: Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells.

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot) and ELISA. Recommended Dilutions: This product has been assayed against 1.0 μg of Pancreatic IsletLysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Dog IgG [H&L] (Goat) and o-Phenylenediamine (OPD) as a substrate. Aworking dilution of 1: 50 to 1: 100 is suggested for this product. This product has beenassayed against 1.5 μg of Pancreatic Islet Lysate [Porcine Pancreas] per lane byImmunoblot (Western) under reducing conditions of SDS-PAGE prior to transfer tonitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG[H&L] (Goat) and Diaminobenzidine (DAB) as a substrate. A working dilution of 1: 50 to1: 200 is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -70 °C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Antigendetails

Target: Pancreatic Islet Lysate