Der Ziege Polyklonal anti-Glucose-6-Phosphate Dehydrogenase Antikörper (ABIN116721) detektiert spezifisch Glucose-6-Phosphate Dehydrogenase in WB und EIA.
Dieser Antikörper reagiert spezifisch mit Proben aus Leuconostoc.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Conjugation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg ofGlucose-6-Phosphate Dehydrogenase [Leuconostoc mesenteroides] in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat)and ABTS (2,2'-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 2,000 to 1: 8,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore with 2.0 mL of deionized water (or equivalent).
Konzentration
95.0 mg/mL (by Refractometry)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Dilute only prior to immediate use . Avoid cycles of freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store vial at -20 °C prior to opening. This product is stable for one month at 2-8 °C as an undiluted liquid. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature.
Target
Glucose-6-Phosphate Dehydrogenase (G6PD)
Andere Bezeichnung
G6PD
Hintergrund
Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency, one benefit of having G6PD deficiency is that it confers a resistance to malaria.Synonyms: Glucose-6-P-Dehydrogenase, Glucose-6-phosphate 1-dehydrogenase