CA2 Antikörper

Produktnummer ABIN116661

Der Kaninchen Polyklonal anti-CA2 Antikörper (ABIN116661) detektiert spezifisch CA2 in WB, IP und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Rind (Kuh).

Kurzübersicht für CA2 Antikörper (ABIN116661)

Target: CA2 (Carbonic Anhydrase II (CA2))

Reaktivität: Human, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CA2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Produktdetails anti-CA2 Antikörper

Spezifität: This antibody detects Carbonic anhydrase 2. Immunoelectrophoresis give a single precipitin arc against purified and partially purified Carbonic anhydrase 2 [bovine erythrocytes].

Aufreinigung: This product was prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: Carbonic Anhydrase II [Bovine Erythrocytes]

Anwendungsinformationen

Applikationshinweise: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayed against 1.0 μg of Carbonic Anhydrase II [BovineErythrocytes] in a standard sandwich ELISA using Peroxidase conjugated affinity purifiedanti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 14,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 85.0 mg/mL (by refractometry)

Buffer: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2, 0.01 % (w/v) Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid.

Details zu CA2

Target: CA2 (Carbonic Anhydrase II (CA2))

Andere Bezeichnung: Carbonic Anhydrase 2

Hintergrund: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20μM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.Synonyms: CA-II, CA2, CAC, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II

Gen-ID: 280740

NCBI Accession: NP_848667

UniProt: P00921