ADH1 Antikörper

Produktnummer ABIN116588

Der Kaninchen Polyklonal anti-ADH1 Antikörper wird verwendet zum Nachweis von ADH1 in Proben von Saccharomyces cerevisiae. Er wurde validiert für WB und EIA.

Kurzübersicht für ADH1 Antikörper (ABIN116588)

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ADH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-ADH1 Antikörper

Spezifität: Assay by Immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alcohol Dehydrogenase [Yeast]. Cross reactivity against Alcohol Dehydrogenase from most fungal sources is likely due to sequence homology as determined by BLAST analysis. Cross reactivity with Alcohol Dehydrogenase from other sources is unknown.

Aufreinigung: Delipidation and defibrination.

Immunogen: Full length Alcohol Dehydrogenase isolated from yeast.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/5,000-1/25,000). Western Blot: 1/500-1/2,000, expect a band approximately 37 kDa in size corresponding tomonomeric alcohol dehdrogenase in the appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 90.0 mg/mL (by refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C. For extended storage aliquot contents and freeze at -20 °C or below.

Details zu ADH1

Target: ADH1 (Alcohol Dehydrogenase 1 (Class I) (ADH1))

Andere Bezeichnung: Alcohol Dehydrogenase 1

Hintergrund: Alcohol dehydrogenase is an isozyme that preferentially catalyzes the conversion of acetaldehyde to acetone. Alcohol dehydrogenase has an apparent molecular weight of 37 kDa (monomer subunit) and forms a homotetramer. This enzyme acts on a variety of primary unbranched aliphatic alcohols and requires 2 bound zinc ions per subunit. Alcohol dehydrogenase shows a cytoplasmic localization. Microheterogeneities may also occur at positions 137, 138, 242-244, and 255 and near position 287.Synonyms: ADC1, ADH1, Alcohol dehydrogenase I, O0947, YADH-1, YOL086C

Gen-ID: 854068

NCBI Accession: NP_014555

UniProt: P00330