C10 / CCL6 Antikörper

Antigen: C10 / CCL6

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Maus

Applikation: Enzyme Immunoassay (EIA), Funktionale Studien (Func), Western Blot (WB)

Produktnummer: ABIN116326

Produktbeschreibung

Produktmerkmale: Synonyms: C-C motif chemokine 6, Small-inducible cytokine A6, Protein C10, Scya6

Swiss-Prot: P27784

Immunogen: Highly pure (>98%) recombinant mC-10.

Beschreibung: C10 belongs to the CC chemokine family and is expressed in myelopoietic bone marrowcultures when stimulated with GM-CSF, M-CSF, IL-3 or IL-4. It signals primarily through theCCR1 receptor. C10 is chemotactic for B cells, CD4+ T cells, monocytes and NK cells andalso exhibits powerful suppressive activity on colony formation by different lineages ofhematopoietic progenitors. The C10 contains the four highly conserved cysteine residuespresent in CC chemokines. The mature protein contains 95 amino acid residues.

Spezifität: Mouse C-10 (MRP-1, CCL6)Add. Information: Centrifuge vial prior to opening!

Anwendungen

Applikationshinweise: Neutralization. ELISA: To detect mC-10 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 ug/ml of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant mC-10. Western Blot: To detect mC-10 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mC-10 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: PP1111P2: 0.1 mg

Buffer: PBS, pH 7.2 without preservatives.Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.

Lagerung: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibodycan be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Forschungsgebiet: Komplementfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar