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Ki-67 Antikörper

MKI67 Reaktivität: Human IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN115328
  • Target Alle Ki-67 (MKI67) Antikörper anzeigen
    Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))
    Reaktivität
    • 216
    • 96
    • 61
    • 16
    • 15
    • 5
    • 2
    • 2
    • 1
    Human
    Wirt
    • 159
    • 52
    • 12
    • 3
    Kaninchen
    Klonalität
    • 150
    • 74
    Polyklonal
    Konjugat
    • 97
    • 15
    • 14
    • 9
    • 9
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    Dieser Ki-67 Antikörper ist unkonjugiert
    Applikation
    • 92
    • 89
    • 67
    • 65
    • 55
    • 52
    • 43
    • 33
    • 30
    • 16
    • 15
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Spezifität
    This antibody reacts with a nuclear antigen present in proliferating human cells. It can be used to evaluate the ki-67 labeling index in various tumors.
    Cellular Localization: Nuclear.
    Aufreinigung
    Purified
    Immunogen
    Synthetic peptide from 62 base pair region of the human Ki-67 antigen.
    Top Product
    Discover our top product MKI67 Primärantikörper
  • Applikationshinweise
    Immunohistochemsistry on Frozen and Formalin-Fixed Paraffin Embedded Sections: Use adilution of 1/25-1/50 in an ABC method (30 minutes at room temperature). Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))
    Andere Bezeichnung
    Ki-67 (MKI67 Produkte)
    Synonyme
    MKI67 antikoerper, kia antikoerper, mki67 antikoerper, MGC132156 antikoerper, D630048A14Rik antikoerper, Ki-67 antikoerper, Ki67 antikoerper, KIA antikoerper, MIB-1 antikoerper, marker of proliferation Ki-67 antikoerper, marker of proliferation Ki-67 L homeolog antikoerper, marker of proliferation Ki-67 S homeolog antikoerper, antigen identified by monoclonal antibody Ki 67 antikoerper, antigen identified by monoclonal antibody Ki-67 antikoerper, nucleolar protein interacting with the FHA domain of MKI67 antikoerper, MKI67 antikoerper, mki67 antikoerper, mki67.L antikoerper, mki67.S antikoerper, Mki67 antikoerper, NIFK antikoerper
    Hintergrund
    Ki-67 antigen is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki-67 gene contains 15 exons. The Ki-67 repeat region, within which there is a 22-amino acid Ki-67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis re-vealed multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quies-cent cells. Immunoblot analysis showed expression of 320- and 359-kD proteins. Sequence analy-sis predicted that the short-lived 2,896- and 3,256-amino acid protein isoforms contain potential nuclear targeting signals, over 200 potential phosphorylation sites, 19 N-myristoylation sites, 3 amidation sites, and numerous PEST sites. Antisense oligonucleotides inhibited cellular prolifera-tion in a dose-dependent manner, suggesting that Ki-67 protein expression may be an absolute requirement for cell proliferation.Synonyms: Ki67 antigen, MKI67, Proliferation marker
    Gen-ID
    4288
    NCBI Accession
    NP_001139438
    UniProt
    P46013
    Pathways
    Glycosaminoglycan Metabolic Process
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