Integrin alpha X (ITGAX) Antikörper (FITC)

Antigen: Integrin alpha X (ITGAX)

Synonyme: CD11C, SLEB6, BR, GPIa, CD49B, VLA-2, VLAA2, VL3A, CD49C, GAPB3, MSK18, VCA-2, VLA3a, GAP-B3, FLJ34631, FLJ34704, IA4, CD49D, MGC90518, FNRA, CD49e, VLA5A, ITGAX, RGD1561123, Cr4, N418, Cd11c, AI449405, CD11c

Klonalität: Monoklonal (R1-2)

Wirt: Ratte

Reaktivität: Maus

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN114227

Produktbeschreibung

Produktmerkmale: Synonyms: Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4, CD49 antigen-like family member D

Weitere Bezeichnung: CD49d / ITGA4

Swiss-Prot: Q00651

Immunogen: Peyers Patch HEV binding lymphoma line (TK1)

Isotyp: IgG2b

Klon: R1-2

Beschreibung: Integrin alpha 4 (also called CD49d) is a 150 kDa protein that possesses a largeextracellular domain involved in ligand binding, a single transmembrane domain, and anintracellular regulatory domain possessing multiple sites for phosphorylation. Integrinalpha 4 forms heterodimers with integrins beta 1 and beta 7. Integrin alpha 4 is expressedon leukocytes and leukocyte precursors, neural crest cells, and developing skeletalmuscles and is essential for embryogenesis, hematopoiesis, and immune responses. Thepresence of integrin alpha 4 promotes cell migration and inhibits cell spreading andcontractility. Integrin alpha 4 function has been implicated in the pathogenesis of multiplediseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis,hepatitis C, and multiple sclerosis, and therefore, modulation of integrin alpha 4 functionhas become an important target for drug discovery.

Spezifität: Antibody ABIN114227 reacts with a4 integrin (mouse CD49d), which helps to mediate cell-celland cell-matrix interactions. Species: mouse. Others not tested.

Synonyme: CD11C, SLEB6, BR, GPIa, CD49B, VLA-2, VLAA2, VL3A, CD49C, GAPB3, MSK18, VCA-2, VLA3a, GAP-B3, FLJ34631, FLJ34704, IA4, CD49D, MGC90518, FNRA, CD49e, VLA5A, ITGAX, RGD1561123, Cr4, N418, Cd11c, AI449405, CD11c

Anwendungen

Protokoll: FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x107 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 106 cells, representing 1 test). 4. To each tube, add 1. 0 µg* of ABIN114226 per 106 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes are protected from light, since most flurochromes arelight sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results: Tissue Distribution by Flow Cytometry Analysis: Mouse Strain: BALB/cCell Concentration: 1x106 cells per testsAntibody Concentration Used: 1. 0 µg/106 cellsIsotypic Control: FITC Rat IgG2bCell Source Percentage of cells stained above control: TK1 cell line 96. 8%Thymus 45. 6%Spleen 88. 0%Bone Marrow 84. 7%Strain Distribution by Flow Cytometry Analysis: Cell Concentration: 1x106 cells per tests

Applikationshinweise: Flow cytometry (see protocol). Immunoprecipitation. Immunohistochemistry. (1,2,3)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.1 mg/ml

Buffer: PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring totalprotein concentration to 4-5 mg/ml.Label: FITC

Lagerung: Store at 4°C. For long term storage, aliquot and freeze unused portion at -20°C in volumesappropriate for single usage. Avoid prolonged exposure to light. Shelf life: one year fromdespatch.

Forschungsgebiet: CD Antigene, Oberflächenrezeptoren der Immunzellen , Zell-/Gewebemarker, Extrazelluläre Matrix, Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Rat anti CD49d (Integrin alpha 4 chain) R1-2

Publikationen

Publikationen: Holzmann, McIntyre, Weissman: "Identification of a murine Peyer's patch--specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4 alpha." in: Cell, Vol. 56, Issue 1, pp. 37-46, 1989 (PubMed).

Holzmann, Weissman: "Peyer's patch-specific lymphocyte homing receptors consist of a VLA-4-like alpha chain associated with either of two integrin beta chains, one of which is novel." in: The EMBO journal, Vol. 8, Issue 6, pp. 1735-41, 1989 (PubMed).