Mitotic Cells Antikörper

Antigen: Mitotic Cells

Klonalität: Monoklonal (A7L6)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN112395

Produktbeschreibung

Immunogen: Total cell lysate of the human bladder carcinoma cell line T24.

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: A7L6

Beschreibung: The life cycle of a eukaryotic cell consists of various phases, two of which canmorphologically and biochemically be identified. Firstly, during mitosis (M-phase), inwhich the cell divides into two identical daughter cells, chromosome condensation andspindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell isreplicated, a process that can be detected using biochemical techniques, such as the BrdUincorporation assay. In between the M- and S-phase two gap phases occur: the G1-phase,the gap between mitosis and the start of DNA replication, and G2-phase, the gap betweencompletion of DNA replication and the onset of mitosis. From G1-phase a cell can leave thecell cycle and enter G0, a 'quiescent' phase. Regulation of the cell cycle predominantlyoccurs at three major control points, which govern the transition from G0 to G1, from G1 toS, and from G2 to M-phase. 8B3G strongly stains mitotic cells and can therefore be used inflow cytometric analyses of cell suspensions to detect the mitotic index. Together with aquantitative DNA staining procedure (e. g. propidium iodide) 8B3G clearly distinguishesthese M-phase cells from cell at other stages of the cell cycle (see figure). Dynamicinformation can be obtained by combining BrdU incorporation with 8B3G staining, whichcan distinguish and quantitate the four major fractions of the cell cycle.

Spezifität: Reacts with human.

Anwendungen

Applikationshinweise: This antibody is suitable for Flow cytometric analyses (1/50-1/100) andImmunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent (1/50-1/100). Not suitable for Immunoblotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: PBS with 0.09% Sodium Azide as preservative.

Lagerung: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar