Desmocollin 3 Antikörper

Produktnummer ABIN112239

Produktdetails anti-Desmocollin 3 Antikörper

Target: Desmocollin 3 (DSC3)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmocollin 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Sequenz: VHGAPFYFSL PNTSPEISRL WSLTKVC

Spezifität: This anibody localizes Dsc3 present in: - all living epidermal layers - in glandular duct cells - in basal matrix cells and outer root sheath of hair follicles - basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), basal layer of bladder urothelium and complex epithelium of trachea - thymic reticulum cells All simple epithelia are negative. Intercalated disks of myocardium are negative. Polypeptide reacting: 109 kDa and 100 kDa polypeptides (desmocollin 3 splice isoforms at pI 5.2) from human epidermal desmosomes. Most tested cultured cell lines derivated from stratified squamous epithelia or squamous cell carcinoma are positive (e.g A-431, HaCat). Does not react with Bovine and Rodent.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Synthetic peptide corresponding to a sequence present on the extracellular anchor domain of Human Desmocollin 3.

Klon: Dsc3-U114

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting (Western). Immunohistochemistry on Frozen Sctions. Immunohistochemistry on Paraffin-Embedded Sections after microwave treatment: 1/10 inPBS ( pH 7.4) for 1h at RT. Recommendations: For better resolution on Frozen Sections preincubation (directly afterfixation) with 0.05-0.2 % Triton X100, for 5-10 min, depending on tissue type, isrecommended (Ref.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in 1 mL distilled water.

Buffer: PBS buffer, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Referenzen für anti-Desmocollin 3 Antikörper (ABIN112239)

Lorén, Cabré, Ojanguren, Domènech, Pedrosa, García-Jaraquemada, Mañosa, Manyé et al.: "Interleukin-10 Enhances the Intestinal Epithelial Barrier in the Presence of Corticosteroids through p38 MAPK Activity in Caco-2 Monolayers: A Possible Mechanism for Steroid Responsiveness in ..." in: PLoS ONE, Vol. 10, Issue 6, pp. e0130921, (2016) (PubMed).

Brenner, Riha, Giessrigl, Thalhammer, Grusch, Krupitza, Stieger, Jäger: "The effect of organic anion-transporting polypeptides 1B1, 1B3 and 2B1 on the antitumor activity of flavopiridol in breast cancer cells." in: International journal of oncology, Vol. 46, Issue 1, pp. 324-32, (2015) (PubMed).

Hamidov, Altendorf-Hofmann, Chen, Settmacher, Petersen, Knösel: "Reduced expression of desmocollin 2 is an independent prognostic biomarker for shorter patients survival in pancreatic ductal adenocarcinoma." in: Journal of clinical pathology, Vol. 64, Issue 11, pp. 990-4, (2012) (PubMed).

Cui, Chen, Yang, Mireskandari, Knösel, Zhang, Kohler, Kunze, Presselt, Petersen: "Diagnostic and prognostic impact of desmocollins in human lung cancer." in: Journal of clinical pathology, Vol. 65, Issue 12, pp. 1100-6, (2012) (PubMed).

Goris, Uhlenbruck, Schwegmann-Wessels, Köhl, Niedorf, Stern, Hewicker-Trautwein, Bals, Taylor, Braun, Bicker, Kietzmann, Herrler: "Differential sensitivity of differentiated epithelial cells to respiratory viruses reveals different viral strategies of host infection." in: Journal of virology, Vol. 83, Issue 4, pp. 1962-8, (2009) (PubMed).

Details zu Desmocollin 3

Target: Desmocollin 3 (DSC3)

Andere Bezeichnung: Desmocollin-3 (DSC3 Produkte)

Synonyme: CDHF3 antikoerper, DSC antikoerper, DSC1 antikoerper, DSC2 antikoerper, DSC4 antikoerper, HT-CP antikoerper, 5430426I24Rik antikoerper, MGC85083 antikoerper, desmocollin 3 antikoerper, desmocollin 3 S homeolog antikoerper, DSC3 antikoerper, Dsc3 antikoerper, dsc3.S antikoerper, dsc3 antikoerper

Hintergrund: Desmocollin 3 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined.Synonyms: CDHF3, Cadherin family member 3, DSC3, DSC4, Desmocollin-4, HT-CP

Gen-ID: 1825

NCBI Accession: NP_001932

UniProt: Q14574