SERPINA3 Antikörper

Produktnummer ABIN111949

Dieser Maus Monoklonal Antikörper detektiert spezifisch SERPINA3 in IHC (p) und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für SERPINA3 Antikörper (ABIN111949)

Target: SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SERPINA3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: ACT14C7

Produktdetails anti-SERPINA3 Antikörper

Spezifität: This antibody recognizes alpha-1 Antichymotrypsin in paraffin embedded and frozen Human cells and tissues. Monocytes and macrophages are recognized in tonsils.

Aufreinigung: Affinity Chromatography

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 1 μg/mL (1/400). Immunohistochemistry on Paraffin Sections: 1 μg/mL (1/400), Microwave pretreatment forantigen retrieval is recommended. Suggested Positive Control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 0.5 mL distilled water.

Konzentration: 0.4 mg/mL

Buffer: PBS buffer pH 7.2 containing 0.1 % Kathon as preservative and 5 mg/mL BSA as stabilizer

Konservierungsmittel: Kathon CG

Vorsichtsmaßnahmen: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C as an undiluted liquid. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Details zu SERPINA3

Target: SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))

Andere Bezeichnung: alpha-1-Antichymotrypsin (ACT)

Hintergrund: The protein is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variation in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease.Synonyms: AACT, Cell growth-inhibiting gene 24/25 protein, GIG24, GIG25, SERPINA3

Gen-ID: 12

NCBI Accession: NP_001076

UniProt: P01011