Macrophages (pan) Antikörper

Antigen: Macrophages

Epitope: pan

Klonalität: Monoklonal (RM1)

Wirt: Maus

Reaktivität: Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN111840

Produktbeschreibung

Produktmerkmale: Synonyms: Rat Macrophage Marker

Weitere Bezeichnung: Macrophages (pan)

Immunogen: Rat Peritoneal Macrophages.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: RM1

Beschreibung: The antigen is found on a subpopullation of most tissue fixed macrophages. It is quitepredominant on red pulp macrophages and interdigitating cells in the outer periarteriolarlymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, andalveolar macrophages. Langerhans cells of the skin and twisted spindle-shaped cells ofthe omentum are detected by the antibody. It is absent from sinusoidal endothelial cellsand parenchymal cells.

Spezifität: This antibody detects monocytes, subpopulation of macrophages, interdigitating cells. The antigen is localized on the surface of cells. It is stable to 2%periodate-lysine-paraformaldehyde fixation. The epitope has not been furthercharacterized. Antigen Distribution: Isolated cells: Positive on monocytes in peripheral blood. Positive on 20-30 % ofperitoneal cells, increasing to 30-40 % after thioglycolate stimulation. Tissue sections: The antigen is found on a subpopulation of most tissue fixedmacrophages. It is quite predominant on red pulp macrophages and interdigitating cells inthe outer periarteriolar lymphatic sheath of the spleen, the inner border of marginal sinus

Anwendungen

Applikationshinweise: Immunohistochemistry on Frozen Sections: 2 µg/ml (1/100). Immunohistochemistry on Paraffin Sections: 20 µg/ml (1/10). Microwave pretreatment forantigen retrieval is recommended. Has been described to work in FACS. Suggested Positive Control: Rat spleen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.2 mg/ml

Buffer: PBS pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.01% Thimerosal as apreservative.Reconstitution: Restore with 0.5 ml distilled water.

Lagerung: Store lyophilized product at 2-8°C. Following reconstitution store (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Forschungsgebiet: Zell-/Gewebemarker, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar