Granzyme K (GZMK) Antikörper

Antigen: Granzyme K (GZMK)

Synonyme: TRYP2

Klonalität: Monoklonal (GM6C3)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Zell-ELISA (cELISA), ELISA

Produktnummer: ABIN108722

Produktbeschreibung

Weitere Bezeichnung: Granzyme K

Immunogen: Our products GM24C3, GM26E7 and GM6C3 were generated by genetic immunisation of BALB/c mice with an immunisation vector containing the granzyme K cDNA. GM24C3, GM26E7 and GM6C3 recognise granzyme K transiently expressed on the cell surface of transfected

Format: Purified

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: GM6C3

Beschreibung: Granzymes are exogenous serine proteases that are stored in the cytotoxic granules of activated T cells and NK cells. Upon target cell contact, the contents of these granules are directionally exocytosed and, with the assistance of perforin, the granzymes enter the cytosol of the target cell. To date, five human granzymes (A, B, H, K, M) have been described at the molecular genetic level. Our products GM24C3, GM26E7 and GM6C3 react with human granzyme K (GrK), a 28 kDa serine protease whose gene is located on chromosome 5q11-12 close to the granzyme A-encoding gene. Like granzyme A, it has a trypsin-like specifity cleaving at the basic residues arginine and lysine. To which extent human granzyme K plays a role in the induction of apoptosis in the target cells remains to be evaluated. However, granzyme K purified from a rat large granular lymphoma cell line (RNK-16) has been shown to induce apoptosis in vitro. High mRNA levels of granzyme K are detected in activated T cells and NK cells but are absent in normal tissues that do not contain high numbers of these cells.

Spezifität: The antibodies do not cross-react with the human granzymes A, B or M (Fig. 1).

Anwendungen

Applikationshinweise: GM6C3 has been tested for the use in flow cytometry, ELISA and cell-ELISA. It is expected that the antibody will be suited for all applications where recognition of the native protein is required. A titration should be performed to determine the optimal

Lagerung: Store the antibody at 4°C. Avoid repeated freezing and thawing.

Forschungsgebiet: Apoptose/Nekrose, Apoptose/Nekrose, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Specificity testing of GM24C3, GM26E7 andGM6C3. BOSC cells weretransiently transfected withexpression vectors for gran-zyme A, B, K and M as well as an irrelevant protein. Ex-pression of the constructs wastested with an anti-tag mono-clonal antibody (B, o

Intracellular detection of granzyme K in humanPBMC. Mononuclear cells from peripheral blood (PBMC)were seperated by Ficoll-Hypaque, fixed andpermeabilised. Cells were incubated with hybridomasupernatant of GM24C3, GM26E7 or GM6C3for 30minutes. Bound anti