NEDD9 Antikörper (AA 82-398)

Produktnummer ABIN108573

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Maus Monoklonal anti-NEDD9 Antikörper wird verwendet zum Nachweis von NEDD9 in Proben von Human, Ratte und Maus. Er wurde validiert für WB, IF, IP, FACS, ICC und IHC (fro).

Kurzübersicht für NEDD9 Antikörper (AA 82-398) (ABIN108573)

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEDD9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 2G9

Produktdetails anti-NEDD9 Antikörper

Bindungsspezifität: AA 82-398

Spezifität: Does not recognize p130 Cas. Not tested on Sin1. This antibody mostly detects HEF1 localized at the focal adhesion sites

Aufreinigung: Protein G

Immunogen: Fusion protein, corresponding to amino acids 82-398 of Human HEF1.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal antibody dilution should be determined by titration, however as a guideline try, WB: 1/2000 - 1/6000. Predicted molecular weight: 93 kDa. IP: 1/1000. ICC/IF: Use at an assay dependent dilution. PubMed: 19376971 IHC-Fr: Use at an assay dependent dilution. PubMed: 19464348 Flow Cyt: Use 1 μg for 106 cells

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Purified antibody containing PBS 0.1 % sodium azide,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Referenzen für anti-NEDD9 Antikörper (ABIN108573)

Tomkiewicz, Herry, Bui, Métayer, Bourdeloux, Barouki, Coumoul: "The aryl hydrocarbon receptor regulates focal adhesion sites through a non-genomic FAK/Src pathway." in: Oncogene, Vol. 32, Issue 14, pp. 1811-20, (2013) (PubMed).

Zhong, Baquiran, Bonakdar, Lees, Ching, Pugacheva, Fabry, ONeill: "NEDD9 stabilizes focal adhesions, increases binding to the extra-cellular matrix and differentially effects 2D versus 3D cell migration." in: PLoS ONE, Vol. 7, Issue 4, pp. e35058, (2012) (PubMed).

ONeill, Golemis: "Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics." in: Molecular and cellular biology, Vol. 21, Issue 15, pp. 5094-108, (2001) (PubMed).

Details zu NEDD9

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Andere Bezeichnung: HEF1

Hintergrund: Synonyms:
CAS L antibody,Cas like docking antibody,CASL antibody,Crk associated substrate related proteinantibody,dJ49G10.2 antibody,dJ761I2.1 antibody,Enhancer of filamentation 1 antibody,HEF 1 antibody,NEDD 9antibody,NEDD9 antibody,NEDD9 protein antibody,Neural cell expressed developmentally down regulated 9antibody,Neural precursor cell expressed developmentally down regulated 9 antibody,NY REN 12 antigenantibody,p105 antibody,Protein NEDD9 antibody

Gen-ID: 4739, 18003, 291044

OMIM: 602265

UniProt: O35177