gamma-Aminobutyric Acid (GABA) Antikörper

Antigen: gamma-Aminobutyric Acid (GABA)

Klonalität: Monoklonal (5A9)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC), ELISA

Produktnummer: ABIN108425

Produktbeschreibung

Weitere Bezeichnung: GABA (gamma-Aminobutyric Acid)

Immunogen: Monoclonal antibody to GABA is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. GABA-coupled to BSA with glutaraldehyde was used as an immunogen

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 5A9

Beschreibung: In the central nervous system GABA is an early neurotransmitter, which can be detected in the developing nervous system. GABA functions as a neurotransmitter also in the peripheral nervous system. Most studies thusfar published on immunolocalization of GABA containing neurons have been made with rabbit polyclonal antibodies. Clearly, there is an increasing need for monoclonal antibodies against neurotransmitters that would enable double immunolocalization studies. Monoclonal antibody 5A9 can be successfully applied with rabbit polyclonal antibodies in double labeling studies as well as a perfect probe for GABA. The antibody has successfully been used in several leading neurobiology laboratories.

Spezifität: The antibody is highly specific to GABA and does not react with other amino acid-BSA conjugates with the exception of weak reaction against b-alanine BSA-conjugate at the highest concentrations used

Anwendungen

Applikationshinweise: Reactivity with paraffin sections. Fluorescence microscopy and other immunohistochemical methods, works also on frozen sections if perfusion fixed. Glutaraldehyde must be included in the fixative with paraffin sections.

Lagerung: This product should be stored at +2 C to +8 C

Forschungsgebiet: Neurotransmitter , Ionenkanäle

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

GABAergic neurons in the reticular nucleus of the thalamus (MAb 5A9, GABA)

Publikationen

Publikationen: Airaksinen MS, Alanen S, Szabat E et al.: "Multiple neurotransmitters in the tuberomammillary nucleus: comparison of rat, mouse, and guinea pig." in: J Comp Neurol, Vol. 323, Issue 1, pp. 103-16, 1992 (PubMed).

Szabat E, Soinila S, Happola O et al.: "A new monoclonal antibody against the GABA-protein conjugate shows immunoreactivity in sensory neurons of the rat." in: Neuroscience, Vol. 47, Issue 2, pp. 409-20, 1992 (PubMed).

Eriksson KS, Panula P: "gamma-Aminobutyric acid in the nervous system of a planarian." in: J Comp Neurol, Vol. 345, Issue 4, pp. 528-36, 1994 (PubMed).

Pitkanen A, Amaral DG: "The distribution of GABAergic cells, fibers, and terminals in the monkey amygdaloid complex: an immunohistochemical and in situ hybridization study." in: J Neurosci, Vol. 14, Issue 4, pp. 2200-24, 1994 (PubMed).

Tuunanen J, Halonen T, Pitkanen A: "Status epilepticus causes selective regional damage and loss of GABAergic neurons in the rat amygdaloid complex." in: Eur J Neurosci, Vol. 8, Issue 12, pp. 2711-25, 1996 (PubMed).

Miettinen M, Pitkanen A, Miettinen R: "Distribution of calretinin-immunoreactivity in the rat entorhinal cortex: coexistence with GABA." in: J Comp Neurol, Vol. 378, Issue 3, pp. 363-78, 1997 (PubMed).

Jongen-Relo AL, Pitkanen A, Amaral DG: "Distribution of GABAergic cells and fibers in the hippocampal formation of the macaque monkey: an immunohistochemical and in situ hybridization study." in: J Comp Neurol, Vol. 408, Issue 2, pp. 237-71, 1999 (PubMed).

Halasy K, Miettinen R, Szabat E et al.: "GABAergic Interneurons are the Major Postsynaptic Targets of Median Raphe Afferents in the Rat Dentate Gyrus." in: Eur J Neurosci, Vol. 4, Issue 2, pp. 144-153, 1992 (PubMed).