Laminin, gamma 1 (LAMC1) Antikörper

Antigen: Laminin, gamma 1 (LAMC1)

Synonyme: LAMB2, MGC87297, B2e, sly, LAMC1, lamb2

Klonalität: Monoklonal (BC17)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC), Immunpräzipitation (IP)

Produktnummer: ABIN108417

Produktbeschreibung

Weitere Bezeichnung: Laminin (gamma1 chain)

Immunogen: Monoclonal antibody (Mab) to laminin g1-chain is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Pepsin-digested laminins from human placenta were used as immunogen.

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: BC17

Beschreibung: Monoclonal Antibody to Human Extracellular Matrix Components

Spezifität: The antibody recognizes the Mr 200,000 g1-chain of human reduced and alkylated laminin produced by various cultured cells, which produce laminins complexed with it. The specificity of Mab BC17 has been confirmed by immunoprecipitation and Elisa by using recombinant g1- chain. The antibody only reacts with human cells and tissues
(further studies are going on).

Anwendungen

Applikationshinweise: No reactivity with paraffin sections. Does not work in western blotting

Lagerung: This product should be stored at +2 C to +8 C

Forschungsgebiet: Extrazelluläre Matrix

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunoprecipitation of the culture medium of human pancreatic carcinoma cells (metabolically labeled with S35Ðmethionine) with MAb BC17 (Laminin g1) (Lane 1). After reduction with dithiotreitol and subsequent treatment with iodoacetamide, only Mr 200 g1 is precipitated (Lane 2)

Publikationen

Publikationen: Geberhiwot T, Wondimu Z, Salo S et al.: "Chain specificity assignment of monoclonal antibodies to human laminins by using recombinant laminin beta1 and gamma1 chains." in: Matrix Biol, Vol. 19, Issue 2, pp. 163-7, 2000 (PubMed).