Nestin (NES) Antikörper

Antigen: Nestin (NES)

Synonyme: FLJ21841, Nbla00170, C78523, ESTM46, AA166324, NES, paranemin

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN104564

Produktbeschreibung

Weitere Bezeichnung: Nestin

Swiss-Prot: P48681

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1484-1500 of human Nestin protein. This affinity purified antibody is directed against human Nestin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Nestin protein from human and macaque (100% homology). Based on protein sequence homology also expect partial reactivity against Nestin homologues from rabbit (94%), mouse (reactivity shown above), rat and hamster (88%), and bovine and dog (82%). Reactivity against homologues from other sources is not known.

Format: Liquid (sterile filtered)

Beschreibung: Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Spezifität: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Anwendungen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 200-220 kDa in size corresponding to Nestin protein by western blotting in the appropriate cell lysate or extract. ELISA: 1:20,000 - 1:80,000. WB: 1:500 - 1:3,000.

Konzentration: 1.1 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Angiogenese, Abstammungsmarker, Zytoskelett

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot using antibodies-online's Affinity Purified anti-Nestin antibody shows detection of a band ~220 kDa corresponding to mouse Nestin (arrowhead). Approximately 30 g of MEF whole cell lysate was separated by SDS-PAGE using a 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2,000 overnight at 4°C. The membrane was then washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX (ABIN102013) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Western blot using antibodies-online's anti-Nestin antibody shows detection of a band ~220 kDa corresponding to human Nestin (arrowhead). Undifferentiated HCN-1A human brain cortical neuron neuronal progenitor lysate (lane 1), or differentiated HCN-1A human brain cortical neuron neuronal progenitor lysate (lane 2) were separated by SDS-PAGE using 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2,000 overnight at 4°C. The membrane was then washed and reacted with a 1:10,000 dilution of peroxidase conjugated affinity purified Gt-a-Rabbit IgG [H&L] MX (ABIN101990) for 45 min at room temperature. Image was captured using film. Other detection systems will yield similar results. Image courtesy of Prof. F.H. Gage of the Salk Institute, San Diego, CA