Ziege anti-Maus Immunoglobulin M (IgM) Antikörper (Biotin)

Antigen: Immunoglobulin M (IgM)

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Maus

Konjugat: Biotin

Applikation: Western Blot (WB), ELISA, Immunhistochemie (IHC), Immunpräzipitation (IP), Fluoreszenzmikroskopie (FM), Durchflusszytometrie (FACS)

Produktnummer: ABIN102676

Produktbeschreibung

Weitere Bezeichnung: Immunoglobulin M

Immunogen: Mouse IgM mu heavy chain

Format: Lyophilized

Spezifität: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose beads followed by solid phase adsorption(s) to remove unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse IgM and Mouse Serum. No reactivity was observed against other mouse heavy or light chain proteins.

Anwendungen

Applikationshinweise: Recommended Dilutions: This product has been assayed against 1.0 ug of Mouse IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:24,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Konzentration: 2.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).