Ziege anti-Human Immunoglobulin M (IgM) Antikörper (Peroxidase)

Antigen: Immunoglobulin M (IgM)

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Konjugat: Peroxidase (POD)

Applikation: Western Blot (WB), ELISA, Immunhistochemie (IHC)

Produktnummer: ABIN102628

Produktbeschreibung

Weitere Bezeichnung: Immunoglobulin M

Immunogen: Human IgM u (mu) heavy chain

Format: Lyophilized

Spezifität: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgM and Human Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.

Anwendungen

Applikationshinweise: ELISA: 1:180,000 WB: 1:2,000 - 1:20,000 IHC: 1:500 - 1:2,500 OTHER: User Optimized

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free. Preservative: 0.01% (w/v) Gentamicin Sulfate. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).