Ziege anti-Kaninchen Immunoglobulin G (IgG) Antikörper (FITC)

Antigen: Immunoglobulin G (IgG)

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Kaninchen

Konjugat: FITC

Applikation: Western Blot (WB), ELISA, Immunhistochemie (IHC), Fluoreszenzmikroskopie (FM), Durchflusszytometrie (FACS)

Produktnummer: ABIN102044

Produktbeschreibung

Weitere Bezeichnung: Immunoglobulin G

Immunogen: Rabbit IgG F(ab) 2 fragment

Format: Lyophilized

Anwendungen

Applikationshinweise: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Konzentration: 2.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free. Preservative: 0.01% (w/v) Thimerisol. Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4.

Lagerung: Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Forschungsgebiet: Sekundärantikörper

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Blakeslee, Baines: "Immunofluorescence using dichlorotriazinylaminofluorescein (DTAF). I. Preparation and fractionation of labelled IgG." in: Journal of immunological methods, Vol. 13, Issue 3-4, pp. 305-20, 1977 (PubMed).