Ziege anti-Human Immunoglobulin G (IgG) Antikörper

Antigen: Immunoglobulin G (IgG)

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Human

Konjugat: Unkonjugiert

Produktnummer: ABIN101520

Produktbeschreibung

Weitere Bezeichnung: Immunoglobulin G

Immunogen: Human IgG whole molecule

Format: Liquid (sterile filtered)

Beschreibung: Affinity Purified anti-Human IgG [H&L] [Goat] Minimum Cross Reactivity to Mouse Serum Proteins

Anwendungen

Applikationshinweise: Suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. ELISA 1:40,000 - 1:160,000. WESTERN BLOT 1:1,000 - 1:5,000. IMMUNOHISTOCHEMISTRY 1:1,000 - 1:2,000.

Konzentration: 2.0 mg/ml (by UV absorbance at 280 nm)

Reinheit: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins. This specificity was confirmed by ELISA at less than 0.8%.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Lagerung: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.

Forschungsgebiet: Sekundärantikörper

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).