Kaninchen anti-Huhn Immunoglobulin G (IgG) Antikörper (Biotin)

Antigen: Immunoglobulin G (IgG)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Huhn

Konjugat: Biotin

Applikation: Western Blot (WB), ELISA, Immunhistochemie (IHC), Immunpräzipitation (IP), Fluoreszenzmikroskopie (FM), Durchflusszytometrie (FACS)

Produktnummer: ABIN101035

Produktbeschreibung

Weitere Bezeichnung: Immunoglobulin G

Immunogen: Chicken IgG whole molecule

Format: Lyophilized

Spezifität: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Chicken IgG and Chicken Serum.

Anwendungen

Applikationshinweise: Recommended Dilutions: This product has been assayed against 1.0 ug of Chicken IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:36,000 to 1:175,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Konzentration: 1.5 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Forschungsgebiet: Sekundärantikörper

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).