Recommended RYK Product (geliefert von: Anmelden zum Anzeigen )

RYK Receptor-Like Tyrosine Kinase (RYK) ELISA Kits
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Kits mit alternativen Reaktivitäten:
Sandwich ELISA
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Produktnummer ABIN4886279
719,71 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
1 ABIN4884386 Sandwich ELISA Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell and Tissue Lysate Anmelden zum Anzeigen
1 ABIN1164624 Anmelden zum Anzeigen


Antigen RYK Receptor-Like Tyrosine Kinase (RYK) ELISA Kits
Epitop pTyr
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(2), (1)
Methodentyp Sandwich ELISA
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails RYK ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck Custom Human Phosphotyrosine RYK ELISA Kit. This assay semi-quantitatively measures phosphotyrosine RYK in lysate samples.
Proben Cell Lysate, Tissue Lysate
Nachweismethode Colorimetric
Analytische Methode Semi-Quantitative
Spezifität The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-RYK.
  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Biotinylated Anti-Phosphotyrosine Antibody
  • Stop Solution
  • Assay Diluent(s)
  • Positive Control Sample
  • Lysis Buffer
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Benötigtes Material
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm


Produktdetails RYK ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
Andere Bezeichnung RYK (RYK ELISA Kit Abstract)
Gen-ID 6259
UniProt P34925
Forschungsgebiet Kinases/Phosphatases, Wnt, Stem Cells, Tyrosine Kinases
Pathways RTK Signalweg, WNT Signalweg, Regulation of Cell Size


Produktdetails RYK ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Probenmenge 100 μL
Plattentyp Pre-coated
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Beschränkungen Nur für Forschungszwecke einsetzbar


Produktdetails RYK ELISA Kit Antigendetails Anwendungsinformationen Bilder zurück nach oben
Lagerung -20 °C
Informationen zur Lagerung Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Haltbarkeit 6 months