GATA3 Antikörper (N-Term)
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- Target Alle GATA3 Antikörper anzeigen
- GATA3 (GATA Binding Protein 3 (GATA3))
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Bindungsspezifität
- N-Term
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Reaktivität
- Human, Maus, Ratte, Rind (Kuh), Schwein, Hund
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser GATA3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Sequenz
- EVTADQPRWV SHH-C
- Spezifität
- This antibody detects GATA3 at N-term. It is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
- Kreuzreaktivität (Details)
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Species reactivity (expected):Mouse, Rat, Dog, Pig, Cow.
Species reactivity (tested):Human. - Aufreinigung
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- Immunogen
- Peptide from the N Terminus of the protein sequence according to NP_001002295.1, NP_002042.1 . Genename: GATA3
- Top Product
- Discover our top product GATA3 Primärantikörper
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- Applikationshinweise
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Peptide ELISA: Antibody detection limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in lysates of cell line MOLT4(calculated MW of 48.0 according to NP_001002295.1 and 47.0 kDa according toNP_002042.1). Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells inthe interfollicular area and in germinal centres. Recommended concentration, 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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Mycoleptodonoides aitchisonii suppresses asthma via Th2 and Th1 cell regulation in an ovalbumin‑induced asthma mouse model." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 11-20, (2018) (PubMed).
: "Erythronium japonicum attenuates histopathological lung abnormalities in a mouse model of ovalbumin-induced asthma." in: International journal of molecular medicine, Vol. 37, Issue 5, pp. 1221-8, (2016) (PubMed).
: "Bacillus subtilis KCTC 11782BP-Produced Alginate Oligosaccharide Effectively Suppresses Asthma via T-Helper Cell Type 2-Related Cytokines." in: PLoS ONE, Vol. 10, Issue 2, pp. e0117524, (2015) (PubMed).
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Mycoleptodonoides aitchisonii suppresses asthma via Th2 and Th1 cell regulation in an ovalbumin‑induced asthma mouse model." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 11-20, (2018) (PubMed).
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- Target
- GATA3 (GATA Binding Protein 3 (GATA3))
- Andere Bezeichnung
- GATA3 (GATA3 Produkte)
- Synonyme
- HDR antikoerper, HDRS antikoerper, Gata-3 antikoerper, jal antikoerper, cb550 antikoerper, gta3 antikoerper, GATA-3 antikoerper, XGATA3 antikoerper, xgata-3 antikoerper, hdr antikoerper, xGATA-3 antikoerper, GATA3 antikoerper, GATA transcription factor 3 antikoerper, T4L20.260 antikoerper, T4L20_260 antikoerper, gata3 antikoerper, ZH-DR antikoerper, zgc:103531 antikoerper, GATA binding protein 3 antikoerper, GATA binding protein 3 S homeolog antikoerper, GATA transcription factor 3 antikoerper, amidase antikoerper, putative amidase antikoerper, GATA-binding protein 3 antikoerper, hematopoietic death receptor antikoerper, GATA3 antikoerper, Gata3 antikoerper, gata3 antikoerper, gata3.S antikoerper, gatA3 antikoerper, hdr antikoerper
- Hintergrund
- The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.Synonyms: GATA-3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3
- Gen-ID
- 2625
- NCBI Accession
- NP_001002295
- UniProt
- P23771
- Pathways
- Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation
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