Nanog Antikörper (Internal Region)

Produktnummer ABIN374591

Produktdetails anti-Nanog Antikörper

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Bindungsspezifität: Internal Region

Reaktivität: Human, Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Nanog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequenz: C-QNQRMKSKRW QKNN

Spezifität: This antibody is specific to NANOG.

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human, Pig.

Aufreinigung: Ammonium sulphate precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_079141.2.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/64000. Western blot: 0.03-0.1 μg/mL. Approx 38 kDa band observed in Human Ovary lysates(calculated MW of 34.8 kDa according to NP_079141.1). Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stemcells are stained for Nanog (green). (See also Ref.2)This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-Nanog Antikörper (ABIN374591)

Montserrat, Ramírez-Bajo, Xia, Sancho-Martinez, Moya-Rull, Miquel-Serra, Yang, Nivet, Cortina, González, Izpisua Belmonte, Campistol: "Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, OCT4 and SOX2." in: The Journal of biological chemistry, Vol. 287, Issue 29, pp. 24131-8, (2012) (PubMed).

Vassena, Montserrat, Carrasco Canal, Aran, de Oñate, Veiga, Izpisua Belmonte: "Accumulation of instability in serial differentiation and reprogramming of parthenogenetic human cells." in: Human molecular genetics, Vol. 21, Issue 15, pp. 3366-73, (2012) (PubMed).

Montserrat, Bahima, Batlle, Häfner, Rodrigues, González, Izpisúa Belmonte: "Generation of pig iPS cells: a model for cell therapy." in: Journal of cardiovascular translational research, Vol. 4, Issue 2, pp. 121-30, (2011) (PubMed).

Montserrat, Garreta, González, Gutiérrez, Eguizábal, Ramos, Borrós, Izpisua Belmonte: "Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system." in: The Journal of biological chemistry, Vol. 286, Issue 14, pp. 12417-28, (2011) (PubMed).

Rodríguez-Pizà, Richaud-Patin, Vassena, González, Barrero, Veiga, Raya, Izpisúa Belmonte: "Reprogramming of human fibroblasts to induced pluripotent stem cells under xeno-free conditions." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 1, pp. 36-44, (2010) (PubMed).

Song, von Figura, Liu, Kraus, Torrice, Dillon, Rudolph-Watabe, Ju, Kestler, Sanoff, Lenhard Rudolph: "Lifestyle impacts on the aging-associated expression of biomarkers of DNA damage and telomere dysfunction in human blood." in: Aging cell, Vol. 9, Issue 4, pp. 607-15, (2010) (PubMed).

Raya, Rodríguez-Pizà, Guenechea, Vassena, Navarro, Barrero, Consiglio, Castellà, Río, Sleep, González, Tiscornia, Garreta, Aasen, Veiga, Verma, Surrallés, Bueren, Izpisúa Belmonte: "Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells." in: Nature, Vol. 460, Issue 7251, pp. 53-9, (2009) (PubMed).

Aasen, Raya, Barrero, Garreta, Consiglio, Gonzalez, Vassena, Bili?, Pekarik, Tiscornia, Edel, Boué, Izpisúa Belmonte: "Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes." in: Nature biotechnology, Vol. 26, Issue 11, pp. 1276-84, (2008) (PubMed).

Details zu Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung: NANOG (NANOG Produkte)

Synonyme: 2410002E02Rik antikoerper, ENK antikoerper, ecat4 antikoerper, NANOG antikoerper, hacp antikoerper, wu:fd19e04 antikoerper, wu:fd20a07 antikoerper, zgc:193933 antikoerper, Nanog homeobox antikoerper, nanog homeobox antikoerper, Nanog antikoerper, NANOG antikoerper, nanog antikoerper

Hintergrund: Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.Synonyms: Homeobox protein NANOG

Gen-ID: 79923, 9606

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance