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Macrophage F4/80 Gen Antikörper (Biotin)

Details zu Produkt Nr. ABIN111850, Anbieter: Anmelden zum Anzeigen
Klonalität (Klon)
Monoklonal ()
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.
Klon Cl:A3-1
Isotyp IgG2b
Spezifität This antibody recognizes the Murine F4/80 antigen, a 160 kDa cell surface glycoprotein. Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Ref.5).
Kreuzreaktivität (Details) Species reactivity (tested):Mouse.
Reinigung Affinity Chromatography on Protein G.
Andere Bezeichnung Macrophage F4/80 Antigen
Hintergrund F4/80 antigen is a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with human EGF module-containing mucinlike hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480
Gen-ID 13733
NCBI Accession NP_034260
UniProt Q61549
Applikationshinweise Flow Cytometry: Use 10 μL of 1/10-1/50 diluted antibody to stain 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 0.5 mg/mL
Buffer PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-Macrophage F4/80 Gen Antikörper (Biotin) (ABIN111850) Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN: BIOTIN (A...
Allgemeine Veröffentlichungen Lin, Faunce, Stacey, Terajewicz, Nakamura, Zhang-Hoover, Kerley, Mucenski, Gordon, Stein-Streilein: "The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance." in: The Journal of experimental medicine, Vol. 201, Issue 10, pp. 1615-25, 2005 (PubMed).

Warschkau, Kiderlen: "A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3409-16, 1999 (PubMed).

Gordon, Lawson, Rabinowitz, Crocker, Morris, Perry: "Antigen markers of macrophage differentiation in murine tissues." in: Current topics in microbiology and immunology, Vol. 181, pp. 1-37, 1992 (PubMed).

Hume, Perry, Gordon: "The mononuclear phagocyte system of the mouse defined by immunohistochemical localisation of antigen F4/80: macrophages associated with epithelia." in: The Anatomical record, Vol. 210, Issue 3, pp. 503-12, 1985 (PubMed).

Lee, Starkey, Gordon: "Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80." in: The Journal of experimental medicine, Vol. 161, Issue 3, pp. 475-89, 1985 (PubMed).

Austyn, Gordon: "F4/80, a monoclonal antibody directed specifically against the mouse macrophage." in: European journal of immunology, Vol. 11, Issue 10, pp. 805-15, 1982 (PubMed).