CD235a/GYPA Antikörper (FITC)
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- Target Alle CD235a/GYPA (GYPA) Antikörper anzeigen
- CD235a/GYPA (GYPA) (Glycophorin A (GYPA))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD235a/GYPA Antikörper ist konjugiert mit FITC
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Applikation
- Flow Cytometry (FACS), Immunofluorescence (IF)
- Produktmerkmale
- Monoclonal Mouse Anti-Human CD235a FITC is recommended for use in flow cytometry.
- Klon
- HI264
- Isotyp
- IgG2a
- Top Product
- Discover our top product GYPA Primärantikörper
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- Applikationshinweise
- It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.
- Kommentare
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Fluorescein isothiocyanate (Molecular Probes) Anti-CD235a HI264 was included in the Seventh International Workshops on Human Leucocyte Differentiation Antigens.
- Probennahme
- 1. Transfer 5 µl of anticoagulated (EDTA) blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 95 µl of PBS (It better that it containing 2% bovine serum albumin) without Fixative mix gently with a vortex mixer. 3. Add 20 µl of CD235a FITC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 4. The recommended negative control is a non-reactive FITC-conjugated antibody of the same isotype. 5. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 6. Add 0, 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 7. Analyse on a flow cytometer, and the readings of the cytometer are recorded, bearing in the mind that the FSC and SSC light dispersion parameters must be on a logarithmic scale.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
- Lagerung
- 4 °C
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Immunophenotyping investigation of minimal residual disease is a useful approach for predicting relapse in acute myeloid leukemia patients." in: Blood, Vol. 90, Issue 6, pp. 2465-70, (1997) (PubMed).
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Immunophenotyping investigation of minimal residual disease is a useful approach for predicting relapse in acute myeloid leukemia patients." in: Blood, Vol. 90, Issue 6, pp. 2465-70, (1997) (PubMed).
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- Target
- CD235a/GYPA (GYPA) (Glycophorin A (GYPA))
- Andere Bezeichnung
- CD235a (GYPA Produkte)
- Synonyme
- GYPA antikoerper, gpa antikoerper, AI853584 antikoerper, CD235a antikoerper, GPA antikoerper, GPErik antikoerper, GPSAT antikoerper, HGpMiV antikoerper, HGpMiXI antikoerper, HGpSta(C) antikoerper, MN antikoerper, MNS antikoerper, PAS-2 antikoerper, glycophorin A (MNS blood group) antikoerper, glycophorin A antikoerper, GYPA antikoerper, Gypa antikoerper
- Pathways
- Maintenance of Protein Location
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