LCK Antikörper (pTyr505)
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- Target Alle LCK Antikörper anzeigen
- LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
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Bindungsspezifität
- pTyr505
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser LCK Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS)
- Kreuzreaktivität
- Ratte (Rattus), Maus
- Produktmerkmale
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. - Aufreinigung
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Human Lck (pY505)
- Klon
- 4-Lck
- Isotyp
- IgG1
- Top Product
- Discover our top product LCK Primärantikörper
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- Kommentare
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Related Products: ABIN968537, ABIN968656, ABIN967389
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store undiluted at -20° C.
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SH2 domain-mediated interaction of inhibitory protein tyrosine kinase Csk with protein tyrosine phosphatase-HSCF." in: Molecular and cellular biology, Vol. 21, Issue 4, pp. 1077-88, (2001) (PubMed).
: "The activated form of the Lck tyrosine protein kinase in cells exposed to hydrogen peroxide is phosphorylated at both Tyr-394 and Tyr-505." in: The Journal of biological chemistry, Vol. 272, Issue 41, pp. 25429-32, (1997) (PubMed).
: "Role of the Lck Src homology 2 and 3 domains in protein tyrosine phosphorylation." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 25003-10, (1996) (PubMed).
: "
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SH2 domain-mediated interaction of inhibitory protein tyrosine kinase Csk with protein tyrosine phosphatase-HSCF." in: Molecular and cellular biology, Vol. 21, Issue 4, pp. 1077-88, (2001) (PubMed).
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- Target
- LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
- Andere Bezeichnung
- Lck (LCK Produkte)
- Synonyme
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zgc:136695 antikoerper, LCK antikoerper, Hck-3 antikoerper, Lsk antikoerper, Lskt antikoerper, p56
antikoerper, p56Lck antikoerper, LSK antikoerper, YT16 antikoerper, p56lck antikoerper, pp58lck antikoerper, P56LCK antikoerper, tkl antikoerper, Lck1 antikoerper, Lcktkr antikoerper, LCK proto-oncogene, Src family tyrosine kinase antikoerper, lymphocyte protein tyrosine kinase antikoerper, lck antikoerper, LCK antikoerper, Lck antikoerper - Hintergrund
- Protein tyrosine phosphorylation is an essential step in the signal transduction cascade leading to T cell antigen receptor (TCR) activation. Lck is a protein kinase and a member of the src family of cytoplasmic protein-tyrosine kinases (PTKs). Members of this family have several common features: 1) unique N-terminal domains, 2) attachment to cellular membranes through a myristylated N-terminus, and 3) homologous SH2, SH3, and catalytic domains. The unique N-terminal domain of Lck interacts with the cytoplasmic tails of the CD4 and CD8 cell surface glycoproteins. CD4 and CD8 bind to surface MHC class II and class I molecules, respectively. Lck is regulated by both kinases and phosphatases. Autophosphorylation at Y394 leads to conformational changes in the catalytic domain, which induces kinase activity. Repression of Lck occurs via phosphorylation at Y505, located near the carboxy-terminus. Phosphorylation of this tyrosine site is mediated by the Csk family of PTKs. Upon phosphorylation at this site, Lck associates with the SH2 domain in the amino-terminus, thus keeping the protein biologically inactive. Lck activity and regulation is critical for activation and development of T cells.
- Molekulargewicht
- 56 kDa
- Pathways
- T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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