The RF8B2 monoclonal antibody specifically binds to the human CXC chemokine receptor, CXCR5. CXCR5 (also known as CD185, BLR-1 NLR and MDR15), a seven transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokine, CXCL13/BLC/BCA-1. In peripheral blood, CXCR5 expression is restricted to B lymphocytes and a small subset of CD4+ and CD8+ lymphocytes. The restricted expression pattern of CXCR5 on B cells and follicular T helper cells (Tfh) suggests that this receptor functions as a regulator of B and T cell migration. Stimulation of T cells with anti-CD3 monoclonal antibody leads to the down-regulation of CXCR5. Detection of CXCR5 expression on human peripheral lymphocytes by biotinylated RF8B2. Human peripheral blood mononuclear cells were stained with Biotin Rat Anti-Human CXCR5 and FITC Mouse Anti-Human CD3 (Cat. No. 555339, Left Panel) or FITC Mouse Anti-Human CD19 (Cat. No. 555412, Right Panel). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using Biotin Rat IgG2b, κ Isotype Control (Cat. No. 555847) and FITC-conjugated mouse IgG2a (Cat. No. 555573) or FITC-conjugated mouse IgG1 (Cat. No. 555748) as isotype controls. The quadrant markers for the bivariate dot plots were set based on the isotype controls.
BD Pharmingen™ Biotin Rat Anti-Human CXCR5 - Biotin - Clone RF8B2 - Isotype Rat IgG2b, κ - Reactivity Hu - 100 Tests
Aufreinigung
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Optimal working dilution should be determined by the investigator.
Probenmenge
20 μL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Lagerung
4 °C
Informationen zur Lagerung
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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