anti-Human WNT9A Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended WNT9A Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) Antikörper
  • zgc:153617
  • wnt-9a
  • Wnt14
  • WNT14
  • WNT-14
  • Wnt family member 9A
  • wingless-type MMTV integration site family, member 9A
  • wingless-type MMTV integration site family member 9A S homeolog
  • WNT9A
  • wnt9a
  • wnt9a.S
  • Wnt9a
Epitop
Internal Region
11
10
9
9
9
8
4
4
2
1
1
1
Reaktivität
Human
77
27
23
1
Wirt
Kaninchen
59
17
Klonalität
Polyklonal
Konjugat
Dieser WNT9A Antikörper ist unkonjugiert
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
38
23
15
13
12
7
2
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1049487
$ 484.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1049488 IHC IHC (p) Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN4886767 IHC (p) WB Rabbit IgG AA 348-363, C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN675533 IF (p) IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 1
1 ABIN1740843 IHC (p) Rabbit IgG Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN1740844 IHC (p) Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN1109515 IHC (p) WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN675542 IHC (p) WB HRP Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN675535 IHC (p) WB Biotin Rabbit IgG Anmelden zum Anzeigen Polyclonal 1
1 ABIN5590981 IHC (p) Rabbit Anmelden zum Anzeigen Polyclonal 0
1 ABIN5590980 IHC (p) Rabbit Anmelden zum Anzeigen Polyclonal 0
1 ABIN2764568 IHC (p) Rabbit Internal Region Anmelden zum Anzeigen Polyclonal 0
1 ABIN2764567 IHC (p) Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0

General

Antigen Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) Antikörper
Epitop Internal Region
(11), (10), (9), (9), (9), (8), (4), (4), (2), (1), (1), (1)
Reaktivität Human
(77), (27), (23), (1)
Wirt Kaninchen
(59), (17)
Klonalität
Polyklonal
Konjugat Dieser WNT9A Antikörper ist unkonjugiert
(3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(38), (23), (15), (13), (12), (7), (2), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-WNT9A Antikörper

Target Details WNT9A Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human WNT9A. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except SCUBE2 (53 % ).
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey, Bat (94%) Mouse, Rat, Hamster, Bovine, Rabbit (88%) Elephant, Dog, Horse (82%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 17 amino acid peptide from internal region of human WNT9A. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey, Bat (94%), Mouse, Rat, Hamster, Bovine, Rabbit (88%), Elephant, Dog, Horse (82%).

Type of Immunogen: Synthetic peptide

Target Details WNT9A

Produktdetails anti-WNT9A Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung WNT14 / WNT9A (WNT9A Antibody Abstract)
Hintergrund Name/Gene ID: WNT9A
Family: WNT

Synonyms: WNT9A, Protein Wnt-14, Protein Wnt-9a, WNT14
Gen-ID 7483
Pathways WNT Signalweg

Anwendungsinformationen

Produktdetails anti-WNT9A Antikörper Target Details WNT9A Handhabung Bilder zurück nach oben
Applikations-hinweise Approved: IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
Kommentare

Target Species of Antibody: Human

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-WNT9A Antikörper Target Details WNT9A Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-WNT9A Antikörper Target Details WNT9A Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (Internal Region) antibody (ABIN1049487) Anti-WNT9A antibody ABIN1049487 IHC staining of human adrenal. Immunohistochemistry ...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (Internal Region) antibody (ABIN1049487) Anti-WNT9A antibody ABIN1049487 IHC staining of human colon. Immunohistochemistry of...