anti-Kaninchen WNT10B Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended WNT10B Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) Antikörper
  • WNT10B
  • Xwnt-10b
  • Xwnt10b
  • wnt-12
  • Wnt12
  • SHFM6
  • WNT-12
  • Xwnt-10
  • wnt10
  • wnt10-A
  • Zwnt[d]
  • wnt10br
  • wnt[d]
  • Wnt family member 10B
  • wingless-type MMTV integration site family, member 10B
  • Wnt family member 10B S homeolog
  • wingless-type MMTV integration site family, member 10b
  • WNT10B
  • wnt10b
  • Wnt10b
  • wnt10b.S
Epitop
Internal Region
15
7
6
5
4
3
2
1
1
Reaktivität
Fledermaus, Hund, Pferd, Human, Affe, Maus, Schwein, Kaninchen, Ratte (Rattus)
62
33
25
3
2
2
2
2
2
2
2
1
Wirt
Kaninchen
47
15
1
Klonalität
Polyklonal
Konjugat
Dieser WNT10B Antikörper ist unkonjugiert
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
40
28
26
18
13
10
6
4
3
2
2
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1049469
$ 484.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN768859 IHC (p) WB Rabbit AA 251-300 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) Antikörper
Epitop Internal Region
(15), (7), (6), (5), (4), (3), (2), (1), (1)
Reaktivität Fledermaus, Hund, Pferd, Human, Affe, Maus, Schwein, Kaninchen, Ratte (Rattus)
(62), (33), (25), (3), (2), (2), (2), (2), (2), (2), (2), (1)
Wirt Kaninchen
(47), (15), (1)
Klonalität
Polyklonal
Konjugat Dieser WNT10B Antikörper ist unkonjugiert
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(40), (28), (26), (18), (13), (10), (6), (4), (3), (2), (2)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-WNT10B Antikörper

Target Details WNT10B Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human WNT10B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Rabbit, Pig (100%) Hamster (94%) Panda (88%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from internal region of human WNT10B. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bat, Horse, Rabbit, Pig (100%), Hamster (94%), Panda (88%).

Type of Immunogen: Synthetic peptide

Target Details WNT10B

Produktdetails anti-WNT10B Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung WNT10B (WNT10B Antibody Abstract)
Hintergrund Name/Gene ID: WNT10B
Family: WNT

Synonyms: WNT10B, Protein Wnt-10b, SHFM6, WNT-12, WNT-10B protein, WNT12, Protein Wnt-12
Gen-ID 7480
Pathways WNT Signalweg, Skeletal Muscle Fiber Development

Anwendungsinformationen

Produktdetails anti-WNT10B Antikörper Target Details WNT10B Handhabung Bilder zurück nach oben
Applikationshinweise Approved: IHC-P (10 - 15 μg/mL)
Kommentare

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-WNT10B Antikörper Target Details WNT10B Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-WNT10B Antikörper Target Details WNT10B Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 10B (WNT10B) (Internal Region) antibody (ABIN1049469) Anti-WNT10B antibody ABIN1049469 IHC staining of human skin. Immunohistochemistry of...