anti-Schaf PKC alpha Antikörper für Flow Cytometry

Recommended PKC alpha Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Protein Kinase C, alpha (PKCa) Antikörper
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C alpha
  • protein kinase C, alpha L homeolog
  • protein kinase C, alpha
  • PRKCA
  • prkca.L
  • prkca
  • Prkca
Reaktivität
Rind (Kuh), Hund, Fisch, Human, Maus, Schwein, Kaninchen, Ratte (Rattus), Schaf
305
218
174
30
25
24
20
20
19
5
3
3
2
1
1
1
1
Wirt
Maus
274
43
2
Klonalität
Monoklonal
Konjugat
Dieser PKC alpha Antikörper ist unkonjugiert
14
13
10
7
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
259
150
135
68
54
49
31
31
26
22
13
5
4
3
2
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN153146
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN4345802 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345811 ELISA FACS ICC IF IHC (fro) IHC (p) FITC Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345813 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345815 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB PerCP Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345816 FACS APC Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345818 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345817 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345821 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345824 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345812 FACS PE Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345814 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345822 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345823 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN4345819 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN5599621 ELISA FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 550 Mouse IgG2a Anmelden zum Anzeigen MC5 0
1 ABIN5599622 ELISA FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 650 Mouse IgG2a Anmelden zum Anzeigen MC5 0

General

Antigen Protein Kinase C, alpha (PKCa) Antikörper
Reaktivität Rind (Kuh), Hund, Fisch, Human, Maus, Schwein, Kaninchen, Ratte (Rattus), Schaf
(305), (218), (174), (30), (25), (24), (20), (20), (19), (5), (3), (3), (2), (1), (1), (1), (1)
Wirt Maus
(274), (43), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser PKC alpha Antikörper ist unkonjugiert
(14), (13), (10), (7), (6), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(259), (150), (135), (68), (54), (49), (31), (31), (26), (22), (13), (5), (4), (3), (2), (1), (1), (1)
Pubmed 14 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-PKC alpha Antikörper

Target Details PKC alpha Anwendungsinformationen Handhabung Referenzen für anti-PKC alpha Antikörper (ABIN153146) Bilder
Spezifität This antibody is capable of detecting PKC beta as well.
Reinigung Protein A purified
Immunogen Purified bovine brain Protein Kinase C
Klon MC5
Isotyp IgG2a
Plasmids, Primers & others

Target Details PKC alpha

Produktdetails anti-PKC alpha Antikörper Anwendungsinformationen Handhabung Referenzen für anti-PKC alpha Antikörper (ABIN153146) Bilder zurück nach oben
Antigen
Andere Bezeichnung PKC alpha (PKCa Antibody Abstract)
Hintergrund Gene Symbol: PRKCA
Gen-ID 5578
UniProt P17252, P04409
Pathways WNT Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

Anwendungsinformationen

Produktdetails anti-PKC alpha Antikörper Target Details PKC alpha Handhabung Referenzen für anti-PKC alpha Antikörper (ABIN153146) Bilder zurück nach oben
Applikations-hinweise Western Blot 1:1000, Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunohistochemistry 1:200-1:500, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunoprecipitation 1:10-1:100, Immunohistochemistry-Paraffin 1:200-1:500, Immunohistochemistry-Frozen 1:200-1:500, RadioimmunoassayThis PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-PKC alpha Antikörper Target Details PKC alpha Anwendungsinformationen Referenzen für anti-PKC alpha Antikörper (ABIN153146) Bilder zurück nach oben
Format Liquid
Konzentration 1.0 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Referenzen für anti-PKC alpha Antikörper (ABIN153146)

Produktdetails anti-PKC alpha Antikörper Target Details PKC alpha Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Xie, Huo, Li, Dai, Xu, Yin: "Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the Müller Cell Notch Signaling Pathway." in: Cell transplantation, Vol. 26, Issue 6, pp. 967-982, 2018 Von den Autoren verwendete Methode: Immunofluorescence (fixed cells) (IF/ICC) (Probematerial (Species): Rat (Rattus)).

Guimarães-Souza, Perche, Morgans, Duvoisin, Calaza: "Fragile X Mental Retardation Protein expression in the retina is regulated by light." in: Experimental eye research, Vol. 146, pp. 72-82, 2016

Zimov, Yazulla: "Novel processes invaginate the pre-synaptic terminal of retinal bipolar cells." in: Cell and tissue research, Vol. 333, Issue 1, pp. 1-16, 2008

Quraishi, Gayet, Morgans, Duvoisin: "Distribution of group-III metabotropic glutamate receptors in the retina." in: The Journal of comparative neurology, Vol. 501, Issue 6, pp. 931-43, 2007

Bayley, Morgans: "Rod bipolar cells and horizontal cells form displaced synaptic contacts with rods in the outer nuclear layer of the nob2 retina." in: The Journal of comparative neurology, Vol. 500, Issue 2, pp. 286-98, 2006

Ng, Parsons, Hughes, Monypenny, Zicha, Gautreau, Arpin, Gschmeissner, Verveer, Bastiaens, Parker: "Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility." in: The EMBO journal, Vol. 20, Issue 11, pp. 2723-41, 2001 (Probematerial (Species): Human). Weitere Details: Immunocytochemistry,Western Blotting,Immunoprecipitation,Immunofluorescence

Cesare, Dekker, Sardini, Parker, McNaughton: "Specific involvement of PKC-epsilon in sensitization of the neuronal response to painful heat." in: Neuron, Vol. 23, Issue 3, pp. 617-24, 1999 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Ricciarelli, Tasinato, Clément, Ozer, Boscoboinik, Azzi: "alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state." in: The Biochemical journal, Vol. 334 ( Pt 1), pp. 243-9, 1998 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Gatti, Robinson: "Unique phosphorylation of protein kinase C-alpha in PC12 cells induces resistance to translocation and down-regulation." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31718-22, 1997 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Clément, Tasinato, Boscoboinik, Azzi: "The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation." in: European journal of biochemistry, Vol. 246, Issue 3, pp. 745-9, 1997 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Gatti, Robinson: "Okadaic acid interferes with phorbol-ester-mediated down-regulation of protein kinase C-alpha, C-delta and C-epsilon." in: European journal of biochemistry, Vol. 249, Issue 1, pp. 92-7, 1997 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Wyss-Coray, Masliah, Toggas, Rockenstein, Brooker, Lee, Mucke: "Dysregulation of signal transduction pathways as a potential mechanism of nervous system alterations in HIV-1 gp120 transgenic mice and humans with HIV-1 encephalitis." in: The Journal of clinical investigation, Vol. 97, Issue 3, pp. 789-98, 1996 Weitere Details: Immunocytochemistry,Immunofluorescence

Escribá, Sastre, García-Sevilla: "Disruption of cellular signaling pathways by daunomycin through destabilization of nonlamellar membrane structures." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16, pp. 7595-9, 1995 (Probematerial (Species): Rat (Rattus)). Weitere Details: Western Blotting

Lacal, Cuadrado, Jones, Trotta, Burstein, Thomson, Pellicer: "Regulation of protein kinase C activity in neuronal differentiation induced by the N-ras oncogene in PC-12 cells." in: Molecular and cellular biology, Vol. 10, Issue 6, pp. 2983-90, 1990 (Probematerial (Species): Human). Weitere Details: Western Blotting

Bilder

Produktdetails anti-PKC alpha Antikörper Target Details PKC alpha Anwendungsinformationen Handhabung Referenzen für anti-PKC alpha Antikörper (ABIN153146) zurück nach oben
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Protein Kinase C, alpha (PKCa) antibody (ABIN153146) Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (MC5) [ABIN153146] - PKC a...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Protein Kinase C, alpha (PKCa) antibody (ABIN153146) Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [ABIN153146] - IHC staining o...