anti-Human FZD6 Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended FZD6 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Frizzled Family Receptor 6 (FZD6) Antikörper
  • zgc:65879
  • zgc:77440
  • fz6
  • frz6
  • Xfrz6
  • frizzled6
  • frizzled-6
  • FZ-6
  • FZ6
  • HFZ6
  • NDNC10
  • Frizzled-6
  • Fz6
  • frizzled class receptor 6
  • frizzled class receptor 6 S homeolog
  • fzd6
  • fzd6.S
  • FZD6
  • Fzd6
Epitop
N-Term
19
14
13
11
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
65
36
13
9
3
3
3
2
2
2
1
1
Wirt
Kaninchen
63
17
6
1
Klonalität
Polyklonal
Konjugat
Dieser FZD6 Antikörper ist unkonjugiert
5
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
62
30
13
13
12
7
6
4
2
1
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN271086
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
12.45388 ABIN1713791 IF (p) IHC (p) WB Rabbit IgG AA 19-50 Anmelden zum Anzeigen Polyclonal 0
12.45388 ABIN4312612 ICC IF IHC IHC (p) Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
12.45388 ABIN4312611 ICC IHC (p) Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN1048613 IHC (p) Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN1048614 ICC IHC (p) Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN122081 IHC (fro) IF IHC (p) Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN1734470 ICC IHC (p) Rabbit IgG N-Term, Extracellular Domain Anmelden zum Anzeigen Polyclonal 0
1 ABIN1734469 IHC (p) Rabbit IgG N-Term, Extracellular Domain Anmelden zum Anzeigen Polyclonal 0
1 ABIN5649969 IF IHC (p) Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
1 ABIN5578512 ICC IHC (p) Rabbit Anmelden zum Anzeigen Polyclonal 0
1 ABIN2765210 ICC IHC (p) IHC Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0
1 ABIN2765211 IHC (p) Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0

General

Antigen Frizzled Family Receptor 6 (FZD6) Antikörper
Epitop N-Term
(19), (14), (13), (11), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(65), (36), (13), (9), (3), (3), (3), (2), (2), (2), (1), (1)
Wirt Kaninchen
(63), (17), (6), (1)
Klonalität
Polyklonal
Konjugat Dieser FZD6 Antikörper ist unkonjugiert
(5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(62), (30), (13), (13), (12), (7), (6), (4), (2), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-FZD6 Antikörper

Target Details FZD6 Anwendungsinformationen Handhabung Bilder
Spezifität Human Frizzled-6. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MTMR10 (47 % ).
Reinigung Immunogen affinity purified
Immunogen Synthetic 17 amino acid peptide from N-terminal extracellular domain of human Frizzled-6.

Target Details FZD6

Produktdetails anti-FZD6 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung Frizzled-6 (FZD6 Antibody Abstract)
Hintergrund Gene Symbol: FZD6
Gen-ID 8323
UniProt O60353
Pathways WNT Signalweg, Tube Formation

Anwendungsinformationen

Produktdetails anti-FZD6 Antikörper Target Details FZD6 Handhabung Bilder zurück nach oben
Applikationshinweise Immunohistochemistry-Paraffin 14 μg/mL
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for Frizzled-6 Antibody (NLS4481)Immunohistochemistry
1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
. Make 4 um sections and place on pre-cleaned and charged microscope slides.
. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each at RT.
. Rehydrate the tissues by washing the slides in 3 changes of 100 % alcohol for 3 minutes each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 3 minutes each at RT.
. Wash the slides in 1 change of 80 % alcohol for 3 minutes at RT.
. Rinse the slides in gentle running distilled water for 5 minutes at RT.
. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.
0) at 99-100 degrees Celciusfor 20 minutes.
. Remove the slides from the heat and let stand in buffer at RT for 20 minutes.
. Rinse the slides in 1X TBS-T for 1 minute at RT.**Do not allow the tissues to dry at any time during the staining procedure**
. Begin the immunostaining by applying a universal protein block for 20 minutes at RT.
. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase steptavidin for 30 minutes at RT.
. Rinse the slide in 1X TBS-T for 1 minute at RT.
. Apply an alkaline phosphatase chromagen substrate for 30 minutes at RT.
. Rinse the slide in distilled water for 1 minute at RT.**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**
. Dehydrate the tissue by washing the slides in 2 changes of 80 % alcohol for 1 minute each at RT.
. Wash the slides in 2 changes of 95 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of 100 % alcohol for 1 minute each at RT.
. Wash the slides in 3 changes of xylene for 1 minute each at RT.
. Apply cover slip.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-FZD6 Antikörper Target Details FZD6 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Bilder

Produktdetails anti-FZD6 Antikörper Target Details FZD6 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Frizzled Family Receptor 6 (FZD6) (N-Term) antibody (ABIN271086) Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4481] - Analysis of anti-Frizz...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Frizzled Family Receptor 6 (FZD6) (N-Term) antibody (ABIN271086) Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4481] - Analysis of anti-Frizz...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Frizzled Family Receptor 6 (FZD6) (N-Term) antibody (ABIN271086) Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4481] - Analysis of anti-Frizz...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Frizzled Family Receptor 6 (FZD6) (N-Term) antibody (ABIN271086) Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4481] - Analysis of anti-Frizz...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Frizzled Family Receptor 6 (FZD6) (N-Term) antibody (ABIN271086) Immunohistochemistry-Paraffin: Frizzled-6 Antibody [NLS4481] - Analysis of anti-Frizz...