anti-Human FZD6 Antikörper für Immunocytochemistry

Recommended FZD6 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Frizzled Family Receptor 6 (FZD6) Antikörper
  • zgc:65879
  • zgc:77440
  • fz6
  • frz6
  • Xfrz6
  • frizzled6
  • frizzled-6
  • FZ-6
  • FZ6
  • HFZ6
  • NDNC10
  • Frizzled-6
  • Fz6
  • frizzled class receptor 6
  • frizzled class receptor 6 S homeolog
  • fzd6
  • fzd6.S
  • FZD6
  • Fzd6
Epitop
N-Term
19
15
13
11
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Affe
74
39
12
8
2
2
2
2
2
2
1
Wirt
Kaninchen
71
17
6
2
Klonalität
Polyklonal
Konjugat
Dieser FZD6 Antikörper ist unkonjugiert
5
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
74
35
20
14
12
8
7
5
4
2
1
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN1048614
$ 484.00
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
10 ABIN6258792 ELISA ICC IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
7 ABIN5578512 ICC IHC (p) Rabbit Anmelden zum Anzeigen Polyclonal 0
4 ABIN1734470 ICC IHC (p) Rabbit IgG N-Term, Extracellular Domain Anmelden zum Anzeigen Polyclonal 0
1 ABIN2886365 ICC IP WB Rabbit AA 167-212 Anmelden zum Anzeigen Polyclonal 0
1 ABIN2765210 ICC IHC (p) Rabbit N-Term Anmelden zum Anzeigen Polyclonal 0

General

Antigen Frizzled Family Receptor 6 (FZD6) Antikörper
Epitop N-Term
(19), (15), (13), (11), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Affe
(74), (39), (12), (8), (2), (2), (2), (2), (2), (2), (1)
Wirt Kaninchen
(71), (17), (6), (2)
Klonalität
Polyklonal
Konjugat Dieser FZD6 Antikörper ist unkonjugiert
(5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(74), (35), (20), (14), (12), (8), (7), (5), (4), (2), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-FZD6 Antikörper

Target Details FZD6 Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human Frizzled-6. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except FZD3 (50 % ).
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%) Mouse, Panda, Bovine, Dog, Horse, Rabbit, Pig (94%) Rat, Opossum, Turkey, Chicken (88%) Elephant, Bat, Lizard (81%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from N-terminal extracellular domain of human Frizzled-6. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%), Mouse, Panda, Bovine, Dog, Horse, Rabbit, Pig (94%), Rat, Opossum, Turkey, Chicken (88%), Elephant, Bat, Lizard (81%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details FZD6

Produktdetails anti-FZD6 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung FZD6 / Frizzled 6 (FZD6 Antibody Abstract)
Hintergrund Name/Gene ID: FZD6
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD6, Frizzled family receptor 6, FZ-6, HFZ6, Frizzled 6, Frizzled-6, Frizzled homolog 6, FZ6, NDNC10
Gen-ID 8323
Pathways WNT Signalweg, Tube Formation

Anwendungsinformationen

Produktdetails anti-FZD6 Antikörper Target Details FZD6 Handhabung Bilder zurück nach oben
Applikations-hinweise Approved: ICC (3 - 6 μg/mL), IHC, IHC-P (6 - 14 μg/mL)
Kommentare

Target Species of Antibody: Human

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-FZD6 Antikörper Target Details FZD6 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-FZD6 Antikörper Target Details FZD6 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Frizzled Family Receptor 6 (FZD6) (N-Term) antibody (ABIN1048614) Anti-Frizzled-6 antibody ABIN1048614 IHC staining of human pancreas. Immunohistochem...
Immunohistochemistry (IHC) image for anti-Frizzled Family Receptor 6 (FZD6) (N-Term) antibody (ABIN1048614) Anti-FZD6 / Frizzled 6 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohi...
Immunocytochemistry (ICC) image for anti-Frizzled Family Receptor 6 (FZD6) (N-Term) antibody (ABIN1048614) Anti-Frizzled-6 antibody immunocytochemistry (ICC) staining of untransfected HEK293 h...