anti-Maus p65 Antikörper für Flow Cytometry

Recommended p65 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antikörper
  • C-Rel
  • NFKB3
  • p65
  • NFkB
  • c-rel
  • zgc:100833
  • REL proto-oncogene, NF-kB subunit
  • RELA proto-oncogene, NF-kB subunit
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel avian reticuloendotheliosis viral oncogene homolog
  • REL
  • RELA
  • Rela
  • Rel
  • rel
Epitop
AA 50-100
131
85
69
63
49
45
41
39
38
37
26
26
25
18
17
15
15
15
13
13
13
12
11
10
8
8
7
6
6
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Schwein, Ratte (Rattus)
1151
667
519
71
56
55
30
25
25
22
13
8
8
6
4
2
1
1
Wirt
Kaninchen
1008
132
17
10
1
Klonalität
Polyklonal
Konjugat
Dieser p65 Antikörper ist unkonjugiert
30
29
23
20
18
18
17
13
12
12
12
8
8
8
8
8
8
8
8
4
4
3
3
2
2
2
2
2
2
1
Applikation
Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
853
445
331
257
206
162
104
91
88
28
19
18
15
12
12
6
6
6
5
5
4
2
2
2
1
Optionen
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Produktnummer ABIN668961
$ 318.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
13.942041 ABIN252655 CyTOF FACS IHC IHC (p) SimWes WB Mouse IgG1 kappa Anmelden zum Anzeigen 112A1021 8
13.942041 ABIN4349897 CyTOF FACS IHC (p) WB Mouse IgG1 kappa Anmelden zum Anzeigen 112A1021 4
13.942041 ABIN4897977 FACS PE Mouse IgG2b AA 456-551, Isoform 1 Anmelden zum Anzeigen 532301 0
13.942041 ABIN4897975 FACS APC Mouse IgG2b AA 456-551, Isoform 1 Anmelden zum Anzeigen 532301 0
13.942041 ABIN4897976 FACS Alexa Fluor 488 Mouse IgG2b AA 456-551, Isoform 1 Anmelden zum Anzeigen 532301 0
13.942041 ABIN4900578 CyTOF FACS ICC WB Mouse IgG2b AA 456-551, Isoform 1 Anmelden zum Anzeigen 532301 0
13.942041 ABIN2658371 FACS APC Mouse IgG2b kappa Anmelden zum Anzeigen 14G10A21 6
13.942041 ABIN2662813 FACS PE Mouse IgG2b kappa Anmelden zum Anzeigen 14G10A21 6
1 ABIN252061 FACS IHC (p) IP WB Biotin Mouse IgG1 kappa Anmelden zum Anzeigen 112A1021 1
1 ABIN1723309 FACS IHC (p) WB Mouse IgG1 kappa AA 526-539 Anmelden zum Anzeigen 112A1021 0
1 ABIN4349890 FACS Alexa Fluor 700 Mouse IgG2b AA 456-551 Anmelden zum Anzeigen 532301 0
1 ABIN4349894 FACS Alexa Fluor 350 Mouse IgG2b AA 456-551 Anmelden zum Anzeigen 532301 0
1 ABIN4349891 FACS Alexa Fluor 647 Mouse IgG2b AA 456-551 Anmelden zum Anzeigen 532301 0
1 ABIN4349892 FACS Alexa Fluor 750 Mouse IgG2b AA 456-551 Anmelden zum Anzeigen 532301 0
1 ABIN4349893 FACS Alexa Fluor 594 Mouse IgG2b AA 456-551 Anmelden zum Anzeigen 532301 0
1 ABIN4349895 FACS Alexa Fluor 405 Mouse IgG2b AA 456-551 Anmelden zum Anzeigen 532301 0
1 ABIN908213 FACS IF (p) Alexa Fluor 488 Rabbit IgG AA 50-100 Anmelden zum Anzeigen Polyclonal 0
1 ABIN908215 FACS IF (p) Alexa Fluor 350 Rabbit IgG AA 50-100 Anmelden zum Anzeigen Polyclonal 0
1 ABIN908216 FACS IF (p) Alexa Fluor 647 Rabbit IgG AA 50-100 Anmelden zum Anzeigen Polyclonal 0
1 ABIN908217 FACS IF (p) Alexa Fluor 555 Rabbit IgG AA 50-100 Anmelden zum Anzeigen Polyclonal 0

General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antikörper
Epitop AA 50-100
(131), (85), (69), (63), (49), (45), (41), (39), (38), (37), (26), (26), (25), (18), (17), (15), (15), (15), (13), (13), (13), (12), (11), (10), (8), (8), (7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Schwein, Ratte (Rattus)
(1151), (667), (519), (71), (56), (55), (30), (25), (25), (22), (13), (8), (8), (6), (4), (2), (1), (1)
Wirt Kaninchen
(1008), (132), (17), (10), (1)
Klonalität
Polyklonal
Konjugat Dieser p65 Antikörper ist unkonjugiert
(30), (29), (23), (20), (18), (18), (17), (13), (12), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Applikation Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(853), (445), (331), (257), (206), (162), (104), (91), (88), (28), (19), (18), (15), (12), (12), (6), (6), (6), (5), (5), (4), (2), (2), (2), (1)
Pubmed 16 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p65 Antikörper

Target Details p65 Anwendungsinformationen Handhabung Referenzen für anti-p65 Antikörper (ABIN668961) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human NFKBp65
Isotyp IgG

Target Details p65

Produktdetails anti-p65 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-p65 Antikörper (ABIN668961) Bilder zurück nach oben
Antigen
Andere Bezeichnung NFKB p65 (NFkBP65 Antibody Abstract)
Hintergrund

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p15, NFKB1/p5, REL and NFKB2/p52 and the heterodimeric p65-p5 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p5 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Subcellular location: Cytoplasm

Synonyms: p65, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA

Gen-ID 5970
UniProt Q04206
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-p65 Antikörper Target Details p65 Handhabung Referenzen für anti-p65 Antikörper (ABIN668961) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Referenzen für anti-p65 Antikörper (ABIN668961) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-p65 Antikörper (ABIN668961)

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Fattori, Borghi, Guazelli, Giroldo, Crespigio, Bussmann, Coelho-Silva, Ludwig, Mazzuco, Casagrande, Verri: "Vinpocetine reduces diclofenac-induced acute kidney injury through inhibition of oxidative stress, apoptosis, cytokine production, and NF-κB activation in mice." in: Pharmacological research, Vol. 120, pp. 10-22, 2018 Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Mouse (Murine)). Weitere Details: Sample: kidney

Ganguly, Wen, Myer, Czech, Sahu, Steinmetz, Raman: "Anti-atherogenic effect of trivalent chromium-loaded CPMV nanoparticles in human aortic smooth muscle cells under hyperglycemic conditions in vitro." in: Nanoscale, Vol. 8, Issue 12, pp. 6542-54, 2016 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Yang, Zhong, Li, Xiong, Wang, Peng, Ye: "Hypothermic machine perfusion increases A20 expression which protects renal cells against ischemia/reperfusion injury by suppressing inflammation, apoptosis and necroptosis." in: International journal of molecular medicine, Vol. 38, Issue 1, pp. 161-71, 2016 Von den Autoren verwendete Methode: Western Blotting (WB), Western Blotting (WB) (Probematerial (Species): Rabbit). Weitere Details: Sample: kidney

Oyagbemi, Omobowale, Asenuga, Adejumobi, Ajibade, Ige, Ogunpolu, Adedapo, Yakubu: "Sodium fluoride induces hypertension and cardiac complications through generation of reactive oxygen species and activation of nuclear factor kappa beta." in: Environmental toxicology, 2016 Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Rat (Rattus)). Weitere Details: Sample: heart, kidney

Xi, Hu, Guo, Wang, Sheng, Han, Yuan, Weng, Xu: "Immunoreactivities of NF-κB, IL-1β and IL-1R in the skin of Chinese brown frog (Rana dybowskii)." in: Acta histochemica, Vol. 119, Issue 1, pp. 64-70, 2016 Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Others). Weitere Details: Sample: skin

Zheng, Liu, Li, Tang, Zhang, Tang: "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." in: Journal of neurosurgery, pp. 1-9, 2016 (Probematerial (Species): Rat (Rattus)). Weitere Details: Sample: brain

Huang, Yang, Wang, Ma, Cui, Huang, Sheng, Weng, Xu: "Immunostimulatory activity of protein hydrolysate from oviductus ranae on macrophage in vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 180234, 2015 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Mouse (Murine)).

Luo, Yang, Tang, Yao, Kong, He, Zhou: "Kaempferol alleviates insulin resistance via hepatic IKK/NF-?B signal in type 2 diabetic rats." in: International immunopharmacology, Vol. 28, Issue 1, pp. 744-50, 2015 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Rat (Rattus)). Weitere Details: Sample: Liver

Zhang, Wu, Song, Wan, Zhang, Tai, Liu: "Effect of hydrogen-rich water on acute peritonitis of rat models." in: International immunopharmacology, Vol. 21, Issue 1, pp. 94-101, 2014 Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Rat (Rattus)).

Niu, Mu, Li, Huang, Yao, Li: "Protective Effects of Chelerythrine Against Lipopolysaccharide-Induced Endotoxic Shock in Mice." in: Inflammation, 2014 Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Mouse (Murine)). Weitere Details: Sample: Lung

Wang, Ma, Su: "NF-?B pathway contributes to cadmium-induced apoptosis of porcine granulosa cells." in: Biological trace element research, Vol. 153, Issue 1-3, pp. 403-10, 2013 Von den Autoren verwendete Methode: Western Blotting (WB) (1:100, Probematerial (Species): Pig (Porcine)).

Song, Wang, Huang, Xiao, Shen, Xu: "NF ?B expression increases and CFTR and MUC1 expression decreases in the endometrium of infertile patients with hydrosalpinx: a comparative study." in: Reproductive biology and endocrinology : RB&E, Vol. 10, pp. 86, 2013 Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Human). Weitere Details: Sample: endometrial tissue

Lin, Yan, Lin, Zhang, Wang, Yang, Zhang: "SIRT1 regulates TNF-α-induced expression of CD40 in 3T3-L1 adipocytes via NF-κB pathway." in: Cytokine, Vol. 60, Issue 2, pp. 447-55, 2012 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Mouse (Murine)).

Zhao, Huang, Wang, Zhong: "Inhibition of NF-kappaB activation by Pyrrolidine dithiocarbamate partially attenuates hippocampal MMP-9 activation and improves cognitive deficits in streptozotocin-induced diabetic rats." in: Behavioural brain research, Vol. 238, pp. 44-7, 2012 Von den Autoren verwendete Methode: Western Blotting (WB), Western Blotting (WB) (Probematerial (Species): Rat (Rattus)). Weitere Details: Sample: brain

Jiang, Tian, Fu, Zhu, Hou: "Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury." in: European journal of pharmacology, Vol. 640, Issue 1-3, pp. 75-81, 2010 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Rat (Rattus)). Weitere Details: Sample: brain

Jing, Piao, Liu, Gao: "Beta2-GPI: a novel factor in the development of hepatocellular carcinoma." in: Journal of cancer research and clinical oncology, Vol. 136, Issue 11, pp. 1671-80, 2010 Von den Autoren verwendete Methode: Immunofluorescence (fixed cells) (IF/ICC) (Probematerial (Species): Human).

Bilder

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung Referenzen für anti-p65 Antikörper (ABIN668961) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit ...
Immunohistochemistry (IHC) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin-embedded rat aortic tissue labeled with Rabbit Anti-NFKB ...
Flow Cytometry (FACS) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated .
Western Blotting (WB) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:5000...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Pol...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Paraformaldehyde-fixed, paraffin embedded rat bladder, Antigen retrieval by boiling i...