anti-Maus TLR9 Antikörper für Western Blotting

Recommended TLR9 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Toll-Like Receptor 9 (TLR9) Antikörper
  • CD289
  • toll like receptor 9
  • toll-like receptor 9
  • TLR9
  • Tlr9
Reaktivität
Maus
263
147
56
36
23
20
7
5
3
2
2
2
2
2
1
1
1
Wirt
Maus
144
123
35
14
4
3
Klonalität
Monoklonal
Konjugat
Dieser TLR9 Antikörper ist konjugiert mit Biotin
26
25
17
8
6
6
5
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Applikation
Detection (D), Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)
228
162
132
109
89
39
37
36
14
7
6
6
6
5
4
3
3
2
2
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN2191786
$ 422.91
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
17.255764 ABIN2191785 D IA IHC (fro) FACS IHC (p) WB FITC Mouse Anmelden zum Anzeigen 5G5 3
17.255764 ABIN2191784 D IA IHC (fro) FACS IHC (p) WB Mouse Anmelden zum Anzeigen 5G5 3
12.755764 ABIN6390108 ELISA WB Rabbit IgG AA 400-480 Anmelden zum Anzeigen Polyclonal 0
12.755764 ABIN5564250 FACS WB Rat IgG2a AA 602-860 Anmelden zum Anzeigen ABM4D70 0
12.755764 ABIN5564253 FACS IHC WB Mouse IgG1 AA 100-290 Anmelden zum Anzeigen ABM1C51 0
12.755764 ABIN5622625 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
12.755764 ABIN2153308 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
10.802607 ABIN6265605 ELISA ICC IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 0
10.802607 ABIN3026222 FACS IHC (p) WB Mouse IgG1, kappa N-Term Anmelden zum Anzeigen TR9541 0
9.302607 ABIN2668281 WB Mouse IgG1 AA 268-284 Anmelden zum Anzeigen 26C593 1
9.302607 ABIN2479769 IHC (fro) WB Rabbit IgG Center Anmelden zum Anzeigen Polyclonal 2
8.5 ABIN2479764 IHC (p) WB Goat IgG N-Term Anmelden zum Anzeigen Polyclonal 1
7.8026066 ABIN1030747 IHC ELISA WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 0
7.8026066 ABIN1031133 ICC ELISA WB Rabbit Middle Region Anmelden zum Anzeigen Polyclonal 0
7.8026066 ABIN5014416 ICC IHC WB Rabbit IgG AA 26-332 Anmelden zum Anzeigen Polyclonal 0
7.8026066 ABIN2903152 IF/ICC IHC IP WB Rabbit AA 26-332 Anmelden zum Anzeigen Polyclonal 0
7 ABIN337069 FACS IHC IHC (fro) IHC (p) WB Mouse IgG1, kappa AA 268-284 Anmelden zum Anzeigen 26C593-2 0
7 ABIN364233 FACS ICC IHC IHC (fro) IHC (p) WB FITC Mouse IgG1 AA 268-284 Anmelden zum Anzeigen 0
7 ABIN625866 ELISA ICC IHC IHC (p) WB Goat AA 204-224, N-Term Anmelden zum Anzeigen Polyclonal 0
7 ABIN214575 IHC IHC (p) WB Rabbit IgG Internal Region Anmelden zum Anzeigen Polyclonal 0

General

Antigen Toll-Like Receptor 9 (TLR9) Antikörper
Reaktivität Maus
(263), (147), (56), (36), (23), (20), (7), (5), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Wirt Maus
(144), (123), (35), (14), (4), (3)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser TLR9 Antikörper ist konjugiert mit Biotin
(26), (25), (17), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Applikation Detection (D), Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)
(228), (162), (132), (109), (89), (39), (37), (36), (14), (7), (6), (6), (6), (5), (4), (3), (3), (2), (2)
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TLR9 Antikörper

Target Details TLR9 Anwendungsinformationen Handhabung Referenzen für anti-TLR9 Antikörper (ABIN2191786) Bilder
Sterilität 0.2 μm filtered
Klon 5G5
Plasmids, Primers & others

Target Details TLR9

Produktdetails anti-TLR9 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-TLR9 Antikörper (ABIN2191786) Bilder zurück nach oben
Antigen
Andere Bezeichnung Toll-Like Receptor 9 (TLR9 Antibody Abstract)
Hintergrund The monoclonal antibody 5G5 reacts with the Toll-like receptor 9 (TLR9, CD289). TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signal via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG- dinucleotides is mediated by TLR9. The monoclonal antibody 5G5 reacts with RAW macrophages and TLR9 transfected HEK293 cells, and is cross reactive with canine TLR9.
Pathways TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-TLR9 Antikörper Target Details TLR9 Handhabung Referenzen für anti-TLR9 Antikörper (ABIN2191786) Bilder zurück nach oben
Applikations-hinweise For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TLR9 Antikörper Target Details TLR9 Anwendungsinformationen Referenzen für anti-TLR9 Antikörper (ABIN2191786) Bilder zurück nach oben
Buffer PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Haltbarkeit 12 months

Referenzen für anti-TLR9 Antikörper (ABIN2191786)

Produktdetails anti-TLR9 Antikörper Target Details TLR9 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." in: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, 2008

Rutz, Metzger, Gellert, Luppa, Lipford, Wagner, Bauer: "Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner." in: European journal of immunology, Vol. 34, Issue 9, pp. 2541-50, 2004

Allgemeine Veröffentlichungen

Ahmad-Nejad, Häcker, Rutz, Bauer, Vabulas, Wagner: "Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments." in: European journal of immunology, Vol. 32, Issue 7, pp. 1958-68, 2002