Produkt kostenlos erhalten?
Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validierenRelevance Score | ABIN | Application | Konjugat | Host | Isotype | Epitope | Hersteller | Clonality | References | Details |
---|---|---|---|---|---|---|---|---|---|---|
17.311333 | ABIN2191786 | D IA IHC (fro) FACS WB | Biotin | Mouse | Anmelden zum Anzeigen | 5G5 | 3 | |||
17.311333 | ABIN2191784 | D IA IHC (fro) FACS IHC (p) WB | Mouse | Anmelden zum Anzeigen | 5G5 | 3 | ||||
8.5 | ABIN2479768 | IHC (fro) WB | Rabbit | IgG | Center | Anmelden zum Anzeigen | Polyclonal | 2 | ||
7 | ABIN337069 | FACS IHC IHC (fro) IHC (p) WB | Mouse | IgG1, kappa | AA 268-284 | Anmelden zum Anzeigen | 26C593-2 | 0 | ||
7 | ABIN364233 | FACS ICC IHC IHC (fro) IHC (p) WB | FITC | Mouse | IgG1 | AA 268-284 | Anmelden zum Anzeigen | 0 | ||
5.5 | ABIN2479766 | IHC (fro) FACS IHC (p) WB | Mouse | IgG2a | Anmelden zum Anzeigen | 5G5 | 3 | |||
4 | ABIN1106226 | EIA IHC (fro) IF IHC (p) WB | FITC | Mouse | IgG2a | AA 1-815, Extracellular Domain, N-Term | Anmelden zum Anzeigen | 5G5 | 7 | |
4 | ABIN1106225 | EIA IHC (fro) IF IHC (p) WB | Biotin | Mouse | IgG2a | AA 1-815, Extracellular Domain, N-Term | Anmelden zum Anzeigen | 5G5 | 7 | |
4 | ABIN951281 | IHC (fro) WB | Rabbit | IgG | Anmelden zum Anzeigen | Polyclonal | 5 | |||
4 | ABIN1723151 | IHC (fro) FACS IHC (p) IHC WB | Mouse | IgG1 kappa | AA 268-284 | Anmelden zum Anzeigen | 26C593-2 | 0 | ||
1 | ABIN265148 | IHC (fro) WB | Rabbit | IgG | C-Term | Anmelden zum Anzeigen | Polyclonal | 5 | ||
1 | ABIN602440 | ELISA FACS IHC IHC (fro) IHC (p) WB | FITC | Mouse | IgG2a | AA 1-815 | Anmelden zum Anzeigen | 0 | ||
1 | ABIN602442 | FACS IHC IHC (fro) IHC (p) WB | Mouse | IgG2a | AA 1-815 | Anmelden zum Anzeigen | 0 | |||
1 | ABIN214906 | FACS IHC IHC (fro) IHC (p) WB | Biotin | Mouse | IgG2a | Anmelden zum Anzeigen | 0 | |||
1 | ABIN602444 | FACS IHC IHC (fro) WB | Biotin | Mouse | IgG2a | Anmelden zum Anzeigen | 0 | |||
1 | ABIN602439 | FACS IHC IHC (fro) IHC (p) WB | FITC | Mouse | IgG2a | Anmelden zum Anzeigen | 0 | |||
1 | ABIN602446 | FACS IHC IHC (fro) WB | Biotin | Mouse | IgG2a | Anmelden zum Anzeigen | 0 | |||
1 | ABIN602441 | FACS IHC IHC (fro) IHC (p) WB | Mouse | IgG2a | Anmelden zum Anzeigen | 0 | ||||
1 | ABIN602445 | FACS IHC IHC (fro) IHC (p) WB | Mouse | IgG2a | Anmelden zum Anzeigen | 0 | ||||
1 | ABIN214905 | FACS IHC IHC (fro) IHC (p) WB | Biotin | Mouse | IgG2a | Anmelden zum Anzeigen | 5G5 | 0 |
General |
|
---|---|
Antigen | Toll-Like Receptor 9 (TLR9) Antikörper |
Reaktivität | Maus Alternativen |
Wirt | Maus Alternativen |
Klonalität (Klon) | |
Konjugat | Dieser TLR9 Antikörper ist konjugiert mit FITC Alternativen |
Applikation |
Detection (D), Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Alternativen
|
![]() |
3 Publikationen vorhanden |
Hersteller | Anmelden zum Anzeigen |
Produktdetails anti-TLR9 AntikörperTarget Details TLR9 Anwendungsinformationen Handhabung Referenzen für anti-TLR9 Antikörper (ABIN2191785) Bilder |
|
Sterilität | 0.2 μm filtered |
Klon | 5G5 |
Plasmids, Primers & others | |
Target Details TLR9Produktdetails anti-TLR9 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-TLR9 Antikörper (ABIN2191785) Bilder zurück nach oben |
|
Antigen | |
Andere Bezeichnung | Toll-Like Receptor 9 (TLR9 Antibody Abstract) |
Hintergrund | The monoclonal antibody 5G5 reacts with the Toll-like receptor 9 (TLR9, CD289). TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signal via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG- dinucleotides is mediated by TLR9. The monoclonal antibody 5G5 reacts with RAW macrophages and TLR9 transfected HEK293 cells, and it is cross reactive with canine TLR9. |
Pathways | TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades |
AnwendungsinformationenProduktdetails anti-TLR9 Antikörper Target Details TLR9 Handhabung Referenzen für anti-TLR9 Antikörper (ABIN2191785) Bilder zurück nach oben |
|
Applikations-hinweise | For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. |
Beschränkungen | Nur für Forschungszwecke einsetzbar |
HandhabungProduktdetails anti-TLR9 Antikörper Target Details TLR9 Anwendungsinformationen Referenzen für anti-TLR9 Antikörper (ABIN2191785) Bilder zurück nach oben |
|
Buffer | PBS, containing 1.0 % bovine serum albumin and 0.02 % sodium azide. |
Konservierungs-mittel | Sodium azide |
Vorsichtsmaßnahmen | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Lagerung | 4 °C |
Informationen zur Lagerung | Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year. |
Haltbarkeit | 12 months |
Referenzen für anti-TLR9 Antikörper (ABIN2191785)Produktdetails anti-TLR9 Antikörper Target Details TLR9 Anwendungsinformationen Handhabung Bilder zurück nach oben |
|
Produkt verwendet in: |
Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." in: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, 2008 Rutz, Metzger, Gellert, Luppa, Lipford, Wagner, Bauer: "Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner." in: European journal of immunology, Vol. 34, Issue 9, pp. 2541-50, 2004 |
Allgemeine Veröffentlichungen |
Ahmad-Nejad, Häcker, Rutz, Bauer, Vabulas, Wagner: "Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments." in: European journal of immunology, Vol. 32, Issue 7, pp. 1958-68, 2002 |