anti-Maus TLR9 Antikörper für Detection

Recommended TLR9 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Toll-Like Receptor 9 (TLR9) Antikörper
  • CD289
  • toll like receptor 9
  • toll-like receptor 9
  • TLR9
  • Tlr9
Reaktivität
Maus
274
166
56
37
24
21
7
5
3
2
2
2
2
2
1
1
Wirt
Maus
145
131
55
14
4
3
Klonalität
Monoklonal
Konjugat
Dieser TLR9 Antikörper ist konjugiert mit Biotin
28
27
19
9
7
6
6
6
6
6
4
4
4
4
3
3
3
3
3
2
2
2
1
1
Applikation
Detection (D), Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)
243
189
134
109
91
39
37
34
15
7
6
6
6
5
4
3
3
2
2
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Mehr erfahren

Produktnummer ABIN2191786
$ 352.43
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN2191785 D IA IHC (fro) FACS IHC (p) WB FITC Mouse Anmelden zum Anzeigen 5G5 3
1 ABIN2191784 D IA IHC (fro) FACS IHC (p) WB Mouse Anmelden zum Anzeigen 5G5 3

General

Antigen Toll-Like Receptor 9 (TLR9) Antikörper
Reaktivität Maus
(274), (166), (56), (37), (24), (21), (7), (5), (3), (2), (2), (2), (2), (2), (1), (1)
Wirt Maus
(145), (131), (55), (14), (4), (3)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser TLR9 Antikörper ist konjugiert mit Biotin
(28), (27), (19), (9), (7), (6), (6), (6), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Applikation Detection (D), Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)
(243), (189), (134), (109), (91), (39), (37), (34), (15), (7), (6), (6), (6), (5), (4), (3), (3), (2), (2)
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TLR9 Antikörper

Target Details TLR9 Anwendungsinformationen Handhabung Referenzen für anti-TLR9 Antikörper (ABIN2191786) Bilder
Sterilität 0.2 μm filtered
Klon 5G5
Plasmids, Primers & others

Target Details TLR9

Produktdetails anti-TLR9 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-TLR9 Antikörper (ABIN2191786) Bilder zurück nach oben
Antigen
Andere Bezeichnung Toll-Like Receptor 9 (TLR9 Antibody Abstract)
Hintergrund The monoclonal antibody 5G5 reacts with the Toll-like receptor 9 (TLR9, CD289). TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signal via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG- dinucleotides is mediated by TLR9. The monoclonal antibody 5G5 reacts with RAW macrophages and TLR9 transfected HEK293 cells, and is cross reactive with canine TLR9.
Pathways TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-TLR9 Antikörper Target Details TLR9 Handhabung Referenzen für anti-TLR9 Antikörper (ABIN2191786) Bilder zurück nach oben
Applikations-hinweise For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TLR9 Antikörper Target Details TLR9 Anwendungsinformationen Referenzen für anti-TLR9 Antikörper (ABIN2191786) Bilder zurück nach oben
Buffer PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
Haltbarkeit 12 months

Referenzen für anti-TLR9 Antikörper (ABIN2191786)

Produktdetails anti-TLR9 Antikörper Target Details TLR9 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." in: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, 2008

Rutz, Metzger, Gellert, Luppa, Lipford, Wagner, Bauer: "Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner." in: European journal of immunology, Vol. 34, Issue 9, pp. 2541-50, 2004

Allgemeine Veröffentlichungen

Ahmad-Nejad, Häcker, Rutz, Bauer, Vabulas, Wagner: "Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments." in: European journal of immunology, Vol. 32, Issue 7, pp. 1958-68, 2002