anti-Human TLR4 Antikörper für Functional Studies

Recommended TLR4 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Toll-Like Receptor 4 (TLR4) Antikörper
  • ARMD10
  • CD284
  • TLR-4
  • TOLL
  • Lps
  • Ly87
  • Ran/M1
  • Rasl2-8
  • toll like receptor 4
  • toll-like receptor 4
  • TLR4
  • Tlr4
Reaktivität
Human
555
274
173
94
72
72
64
26
12
9
4
2
1
1
Wirt
Maus
339
272
47
8
Klonalität
Monoklonal
Konjugat
Dieser TLR4 Antikörper ist konjugiert mit FITC
48
47
46
23
15
14
13
10
9
9
8
6
6
6
6
6
6
6
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Functional Studies (Func), Flow Cytometry (FACS)
392
342
187
137
130
124
98
92
34
32
27
26
25
21
20
16
14
8
7
4
4
3
2
1
1
1
Optionen
Hersteller
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Produktnummer ABIN2191938
$ 558.80
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
21.755764 ABIN2191939 Func FACS PE Mouse IgG2a Anmelden zum Anzeigen HTA125 8
21.755764 ABIN2191941 Func FACS Biotin Mouse IgG2a Anmelden zum Anzeigen HTA125 8
21.755764 ABIN2191940 Func FACS PE Mouse IgG2a Anmelden zum Anzeigen HTA125 8
21.755764 ABIN2191937 Func FACS Mouse IgG2a Anmelden zum Anzeigen HTA125 8
12.755764 ABIN2153540 Func Mouse IgG2a kappa Anmelden zum Anzeigen HTA125 0
12.755764 ABIN2192090 Anta Func FACS Mouse Anmelden zum Anzeigen 3C3 0
5.5 ABIN2479755 Func FACS IP WB Mouse IgG2a Anmelden zum Anzeigen HTA125 4
4 ABIN119330 Func FACS IP WB Mouse IgG2a Anmelden zum Anzeigen HTA125 2
1 ABIN198147 Func FACS IP ELISA Biotin Mouse IgG2a Anmelden zum Anzeigen 0
1 ABIN198151 Func FACS IP Mouse IgG2a Anmelden zum Anzeigen 0
1 ABIN1106216 Func FACS Mouse IgG1 Anmelden zum Anzeigen 7-00E-003 0
1 ABIN6252558 FACS Func IF IHC (p) Mouse IgG2a, kappa Anmelden zum Anzeigen TLR4-230 0
1 ABIN217676 Func FACS IP WB Mouse IgG2a Anmelden zum Anzeigen 0
1 ABIN1106215 Func FACS Mouse IgG1 Anmelden zum Anzeigen 3C3 0
1 ABIN3026986 Func FACS IF IHC (p) Mouse IgG2a, kappa Anmelden zum Anzeigen TLR4-230 0
1 ABIN6297482 FACS Func IF IHC Mouse Anmelden zum Anzeigen 0
1 ABIN3026987 Func FACS IF IHC (p) Mouse IgG2a, kappa Anmelden zum Anzeigen TLR4-230 0
1 ABIN2567453 Func FACS Mouse IgG2a kappa Anmelden zum Anzeigen HTA125 0
1 ABIN2567452 Func FACS Mouse IgG2a kappa Anmelden zum Anzeigen HTA125 0
1 ABIN187556 Func FACS IP Mouse IgG2a Anmelden zum Anzeigen HTA125 0

General

Antigen Toll-Like Receptor 4 (TLR4) Antikörper
Reaktivität Human
(555), (274), (173), (94), (72), (72), (64), (26), (12), (9), (4), (2), (1), (1)
Wirt Maus
(339), (272), (47), (8)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser TLR4 Antikörper ist konjugiert mit FITC
(48), (47), (46), (23), (15), (14), (13), (10), (9), (9), (8), (6), (6), (6), (6), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Functional Studies (Func), Flow Cytometry (FACS)
(392), (342), (187), (137), (130), (124), (98), (92), (34), (32), (27), (26), (25), (21), (20), (16), (14), (8), (7), (4), (4), (3), (2), (1), (1), (1)
Pubmed 8 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-TLR4 Antikörper

Target Details TLR4 Anwendungsinformationen Handhabung Referenzen für anti-TLR4 Antikörper (ABIN2191938) Bilder
Kreuzreaktivität (Details) Cross reactivity: Canine : Yes, Cynomolgus monkey : Yes, Rhesus monkey : Yes, Marmoset monkey : Yes
Sterilität 0.2 μm filtered
Klon HTA125
Isotyp IgG2a
Plasmids, Primers & others

Target Details TLR4

Produktdetails anti-TLR4 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-TLR4 Antikörper (ABIN2191938) Bilder zurück nach oben
Antigen
Andere Bezeichnung Toll-Like Receptor 4 (TLR4 Antibody Abstract)
Hintergrund Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB- dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single α-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses. Toll-like receptor 4 (TLR4, CD284) has been identified, next to MD-2 and CD14, as a receptor that is central to the innate immune response to LPS of Gram-negative bacteria. TLR4 is unique among TLRs in its ability to activate two distinct signaling pathways, one pathway is activated by the adaptors TIRAP (Toll/interleukin-1- receptor (TIR)-domain-containing adaptor protein) and MyD88, which leads to the induction of pro-inflammatory cytokines. The second pathway is activated by the adaptors TRIF (TIR- domaincontaining adaptor protein inducing interferon-β) and TRAM (TRIFrelated adaptor molecule), which leads to the induction of type I interferons. The monoclonal antibody HTA125 is a TLR4 function-blocking antibody. HTA125 recognizes preferentially human TLR4 that is associated with MD-2. Aliases TLR4, CD284, ARMD10 Immunogen BALB/c mice were immunized with the Ba/F3 line expressing TLR4.
Pathways TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-TLR4 Antikörper Target Details TLR4 Handhabung Referenzen für anti-TLR4 Antikörper (ABIN2191938) Bilder zurück nach oben
Applikations-hinweise For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Macrophages control Negative HEK293 cells control
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-TLR4 Antikörper Target Details TLR4 Anwendungsinformationen Referenzen für anti-TLR4 Antikörper (ABIN2191938) Bilder zurück nach oben
Buffer PBS, containing 1 % bovine serum albumin and 0.02 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

Referenzen für anti-TLR4 Antikörper (ABIN2191938)

Produktdetails anti-TLR4 Antikörper Target Details TLR4 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Brüll, Mensink, van den Hurk, Duijvestijn, Plat: "TLR2 activation is essential to induce a Th1 shift in human peripheral blood mononuclear cells by plant stanols and plant sterols." in: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 2951-8, 2010

Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." in: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, 2008

Triantafilou, Morath, Mackie, Hartung, Triantafilou: "Lateral diffusion of Toll-like receptors reveals that they are transiently confined within lipid rafts on the plasma membrane." in: Journal of cell science, Vol. 117, Issue Pt 17, pp. 4007-14, 2004

Walton, Hsieh, Gharavi, Wang, Wang, Yeh, Cole, Berliner et al.: "Receptors involved in the oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine-mediated synthesis of interleukin-8. A role for Toll-like receptor 4 and a ..." in: The Journal of biological chemistry, Vol. 278, Issue 32, pp. 29661-6, 2003

Tabeta, Yamazaki, Akashi, Miyake, Kumada, Umemoto, Yoshie: "Toll-like receptors confer responsiveness to lipopolysaccharide from Porphyromonas gingivalis in human gingival fibroblasts." in: Infection and immunity, Vol. 68, Issue 6, pp. 3731-5, 2000

Shimazu, Akashi, Ogata, Nagai, Fukudome, Miyake, Kimoto: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, 1999

Allgemeine Veröffentlichungen

Wang, Warris, Ellingsen, Jørgensen, Flo, Espevik, Solberg, Verweij, Aasen: "Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae." in: Infection and immunity, Vol. 69, Issue 4, pp. 2402-6, 2001

Akashi, Ogata, Kirikae, Kirikae, Kawasaki, Nishijima, Shimazu, Nagai, Fukudome, Kimoto, Miyake: "Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2." in: Biochemical and biophysical research communications, Vol. 268, Issue 1, pp. 172-7, 2000