anti-Maus TLR1 Antikörper für Immunohistochemistry

Recommended TLR1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen

Toll-Like Receptor 1 (TLR1) Antikörper
Synonyme für dieses Antigen anzeigen

CD281
TIL
TIL. LPRS5
rsc786
toll like receptor 1
toll-like receptor 1
TLR1
Tlr1

Epitop

AA 350-404
Alternativen

Reaktivität

Human, Maus, Ratte (Rattus)
Alternativen

178

Wirt

Kaninchen
Alternativen

140

Klonalität

Konjugat

Dieser TLR1 Antikörper ist unkonjugiert
Alternativen

Applikation

ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
Alternativen

131

Optionen

Hersteller

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Western Blotting (WB) image for anti-Toll-Like Receptor 1 (TLR1) (AA 350-404) antibody (ABIN5693146)

Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 1 (TLR1) (AA 350-404) antibody (ABIN5693146)

Flow Cytometry (FACS) image for anti-Toll-Like Receptor 1 (TLR1) (AA 350-404) antibody (ABIN5693146)

Produktnummer ABIN5693146

$ 280.00
Zzgl. Versandkosten $45.00

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Preis

100 μg

$280.00

Product detail page

Relevance Score	ABIN	Application	Konjugat	Host	Isotype	Epitope	Hersteller	Clonality	References
8.664236	ABIN959418	FACS IHC IHC (p) WB		Rabbit	IgG	AA 400-450	Anmelden zum Anzeigen	Polyclonal	0
8.664236	ABIN214527	IHC IHC (p) WB		Rabbit	IgG	N-Term	Anmelden zum Anzeigen	Polyclonal	0
8.664236	ABIN3181539	IHC		Rabbit	IgG		Anmelden zum Anzeigen	Polyclonal	0
8.664236	ABIN3181537	IHC		Rabbit	IgG		Anmelden zum Anzeigen	Polyclonal	0
8.664236	ABIN5960090	IHC		Rabbit	IgG		Anmelden zum Anzeigen	Polyclonal	0
8.664236	ABIN1031619	IHC ELISA WB		Rabbit		N-Term	Anmelden zum Anzeigen	Polyclonal	0
8.664236	ABIN3029159	IHC ELISA WB		Rabbit	Ig Fraction	AA 764-795	Anmelden zum Anzeigen	Polyclonal	0
8.664236	ABIN2427609	IHC ELISA WB		Rabbit	IgG		Anmelden zum Anzeigen	Polyclonal	0
1	ABIN252519	FACS IHC IHC (p) WB		Rabbit			Anmelden zum Anzeigen	Polyclonal	11
1	ABIN1003315	IHC ELISA WB		Rabbit	IgG	N-Term	Anmelden zum Anzeigen	Polyclonal	4
1	ABIN3023655	IHC WB		Rabbit	IgG		Anmelden zum Anzeigen	Polyclonal	0
1	ABIN1871629	ICC IHC WB		Rabbit	IgG	AA 25-134	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN6265596	ELISA IHC WB		Rabbit	IgG		Anmelden zum Anzeigen	Polyclonal	0
1	ABIN1942114	IHC ELISA WB	PE	Rabbit	IgG	AA 764-795	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN1942110	IHC ELISA WB	Biotin	Rabbit	IgG	AA 764-795	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN1942111	IHC ELISA WB	Alkaline Phosphatase (AP)	Rabbit	IgG	AA 764-795	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN1942113	IHC ELISA WB	APC	Rabbit	IgG	AA 764-795	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN1942112	IHC ELISA WB	FITC	Rabbit	IgG	AA 764-795	Anmelden zum Anzeigen	Polyclonal	0
1	ABIN2888061	IF IHC WB		Rabbit	IgG		Anmelden zum Anzeigen	Polyclonal	0
1	ABIN1942109	IHC ELISA WB	HRP	Rabbit	IgG	AA 764-795	Anmelden zum Anzeigen	Polyclonal	0

Weitere Produktkategorien zu TLR1 Antikörper

anti-TLR1 Antikörper für Immunohistochemistry mit den meisten Publikationen

Human Polyclonal TLR1 Primary Antibody für FACS, IHC - ABIN252519 (11 Publications): Palladino, Savarese, Chapman, Dughi, Plaska: Localization of Toll-like receptors on epididymal epithelial cells and spermatozoa. in American journal of reproductive immunology (New York, N.Y. : 1989) 2008 (PubMed)
Human Polyclonal TLR1 Primary Antibody für IHC, ELISA - ABIN1003315 (4 Publications): Takeda, Kaisho, Akira: Toll-like receptors. in Annual review of immunology 2003 (PubMed)

General
Antigen	Toll-Like Receptor 1 (TLR1) Antikörper
Epitop	AA 350-404 Alternativen
	(26), (12), (11), (7), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reaktivität	Human, Maus, Ratte (Rattus) Alternativen
	(178), (90), (41), (1)
Wirt	Kaninchen Alternativen
	(140), (47), (17), (1)
Klonalität	Polyklonal
Konjugat	Dieser TLR1 Antikörper ist unkonjugiert Alternativen
	(18), (17), (16), (5), (5), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation	ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB) Alternativen (131), (77), (74), (51), (37), (14), (13), (13), (13), (12), (11), (7), (6), (3), (2)
Hersteller	Anmelden zum Anzeigen
Produktdetails anti-TLR1 Antikörper Target Details TLR1 Anwendungsinformationen Handhabung Bilder
Marke	Picoband™
Kreuzreaktivität (Details)	No cross reactivity with other proteins.
Produktmerkmale	Rabbit IgG polyclonal antibody for TLR1 detection. Tested with WB, IHC-P, IHC-F, ICC, FCM, Direct ELISA in Human,Mouse,Rat.
Immunogen	E. coli-derived human TLR1 recombinant protein (Position: F350-D404).
Plasmids, Primers & others
Target Details TLR1 Produktdetails anti-TLR1 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung	TLR1 (TLR1 Antibody Abstract)
Hintergrund	Synonyms: Toll-like receptor 1, Toll/interleukin-1 receptor-like protein, TIL, CD281, TLR1, KIAA0012 Tissue Specificity: Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. Background: Toll-like receptor 1 (TLR1), also called TIL or CD281 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. This gene is mapped to 4p14 by fluorescence in situ hybridization. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene.
UniProt	Q15399
Pathways	TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Anwendungsinformationen Produktdetails anti-TLR1 Antikörper Target Details TLR1 Handhabung Bilder zurück nach oben
Applikations-hinweise	Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC. Application Details: Western blot, 0.1-0.5&mu,g/mL Immunohistochemistry(Paraffin-embedded Section), 0.5-1&mu,g/mL Immunohistochemistry(Frozen Section), 0.5-1&mu,g/mL Immunocytochemistry, 0.5-1&mu,g/mL Flow Cytometry, 1-3 μg/1x10⁶ cells Direct ELISA, 0.1-0.5&mu,g/mL
Beschränkungen	Nur für Forschungszwecke einsetzbar
Handhabung Produktdetails anti-TLR1 Antikörper Target Details TLR1 Anwendungsinformationen Bilder zurück nach oben
Format	Lyophilized
Rekonstitution	Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Buffer	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.
Konservierungs-mittel	Sodium azide
Vorsichtsmaßnahmen	This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung	4 °C,-20 °C
Informationen zur Lagerung	At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Bilder Produktdetails anti-TLR1 Antikörper Target Details TLR1 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers	Western blot analysis of TLR1 using anti-TLR1 antibody . Electrophoresis was perform... IHC analysis of TLR1 using anti-TLR1 antibody . TLR1 was detected in paraffin-embedde... IHC analysis of TLR1 using anti-TLR1 antibody . TLR1 was detected in paraffin-embedde... IHC analysis of TLR1 using anti-TLR1 antibody . TLR1 was detected in paraffin-embedde... Flow Cytometry analysis of K562 cells using anti-TLR1 antibody . Overlay histogram sh... Flow Cytometry analysis of THP-1 cells using anti-TLR1 antibody . Overlay histogram s... Images for product: anti-Toll-Like Receptor 1 (TLR1) (AA 350-404) antibody Western blot analysis of TLR1 using anti-TLR1 antibody . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each Lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: mouse small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR1 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR1 at approximately 90KD. The expected band size for TLR1 is at 90KD. IHC analysis of TLR1 using anti-TLR1 antibody . TLR1 was detected in paraffin-embedded section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-TLR1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. IHC analysis of TLR1 using anti-TLR1 antibody . TLR1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-TLR1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. IHC analysis of TLR1 using anti-TLR1 antibody . TLR1 was detected in paraffin-embedded section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-TLR1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Flow Cytometry analysis of K562 cells using anti-TLR1 antibody . Overlay histogram showing K562 cells stained with (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLR1 Antibody (,1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. Flow Cytometry analysis of THP-1 cells using anti-TLR1 antibody . Overlay histogram showing THP-1 cells stained with (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLR1 Antibody (,1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Recommended TLR1 Antibody (geliefert von: Anmelden zum Anzeigen )

General

Produktdetails anti-TLR1 Antikörper

Target Details TLR1

Anwendungsinformationen

Handhabung

Bilder