anti-Maus TANK-Binding Kinase 1 Antikörper für Intracellular Staining

Recommended TANK-Binding Kinase 1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
TANK-Binding Kinase 1 (TBK1) Antikörper
  • nak
  • t2k
  • TBK1
  • wu:fk70c05
  • zgc:136548
  • NAK
  • T2K
  • 1200008B05Rik
  • AI462036
  • AW048562
  • TANK binding kinase 1 L homeolog
  • TANK binding kinase 1
  • TANK-binding kinase 1
  • tbk1.L
  • TBK1
  • tbk1
  • Tbk1
Epitop
pSer172
20
19
17
10
7
7
4
4
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
133
86
50
30
29
10
10
9
8
5
5
4
3
2
2
2
2
1
1
Wirt
Maus
103
36
13
Klonalität
Monoklonal
Konjugat
Alexa Fluor 647
10
10
9
8
6
5
3
2
1
1
1
1
1
1
1
1
1
1
Applikation
Intracellular Staining (ICS)
134
65
63
34
24
17
9
5
3
2
2
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Produktnummer ABIN1177215
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1177216 ICS Alexa Fluor 488 Mouse IgG1, kappa pSer172 Anmelden zum Anzeigen J133-587 1
1 ABIN1177214 ICS PE Mouse IgG1, kappa pSer172 Anmelden zum Anzeigen J133-587 0

General

Antigen TANK-Binding Kinase 1 (TBK1) Antikörper
Epitop pSer172
(20), (19), (17), (10), (7), (7), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(133), (86), (50), (30), (29), (10), (10), (9), (8), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1)
Wirt Maus
(103), (36), (13)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Alexa Fluor 647
(10), (10), (9), (8), (6), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Intracellular Staining (ICS)
(134), (65), (63), (34), (24), (17), (9), (5), (3), (2), (2), (2), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Marke BD Phosflow™
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human TBK1
Klon J133-587
Isotyp IgG1, kappa

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Andere Bezeichnung TBK1 (TBK1 Antibody Abstract)
Hintergrund NF-kB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. In most cells, NF-kB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IkBalpha, IkBbeta, and IkBepsilon. Stimulation induces the release, activation, and nuclear translocation of NF-kB. Release of NF-kB results from the phosphorylation and proteolytic degradation of the IkB proteins. Two cytokine-inducible IkappaB kinases (IKKa and IKKb) phosphorylate and target the IkB proteins for degradation via the ubiquitin pathway. IKKg/NEMO, a third member of the IKK complex, functions as a regulatory subunit and interacts directly with IKKb. TBK1 (TANK-binding kinase 1, also known as T2K or NAK), a protein of 729 amino acids, is another member of the IKK family of kinases regulating NF-kB downstream of the tumor necrosis factor and Toll-like receptor pathways. TBK1 forms a complex with the adaptor proteins TANK (TRAF-associated NF-kB activator) and TRAF2 (TNF-receptor-associated factor 2), and this oligomer is required for activation and phosphorylation of TBK1 at serine 172 (S172). The J133-587 monoclonal antibody recognizes the phosphorylated S172 of human TBK1. Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7 because we have been unable to detect endogenous TBK1 (pS172) by western blotting of lysates from activated cell lines (HeLa activated with PMA and A431 activated with PDGF). In the transfectants, the over-expression of TBK1 is necessary for phosphorylation of IRF-7, but the presence of IRF-7 does not affect TBK1 expression or phosphorylation. We confirmed that mAb J133-587 does not cross-react with IRF-7 by ELISA.
Synonyms: T2K, NAK
Pathways TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Probenmenge 20 μL
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Viatour, Merville, Bours, Chariot: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." in: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, 2005