anti-Human IRF7 Antikörper für Intracellular Staining

Recommended IRF7 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Interferon Regulatory Factor 7 (IRF7) Antikörper
  • wu:fl24c03
  • zgc:66043
  • zgc:77947
  • IRF7
  • irf7
  • IRF-7H
  • IRF7A
  • IRF7B
  • IRF7C
  • IRF7H
  • interferon regulatory factor 7
  • irf7
  • IRF7
  • Irf7
Epitop
pSer477
30
23
15
15
15
15
6
5
4
4
3
3
2
1
1
Reaktivität
Human
152
58
55
3
3
3
2
2
2
1
1
Wirt
Maus
108
44
Klonalität
Monoklonal
Konjugat
Dieser IRF7 Antikörper ist konjugiert mit Alexa Fluor 488
7
6
6
6
6
6
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Intracellular Staining (ICS)
108
54
27
27
26
10
6
4
4
2
2
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN1177072
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1176907 ICS Alexa Fluor 488 Mouse IgG1, kappa Anmelden zum Anzeigen K40-321 1
1 ABIN1177070 ICS PE Mouse IgG1, kappa pSer477 Anmelden zum Anzeigen K47-671 1
1 ABIN1177071 ICS Alexa Fluor 647 Mouse IgG1, kappa pSer477 Anmelden zum Anzeigen K47-671 1
1 ABIN1176906 ICS PE Mouse IgG1, kappa Anmelden zum Anzeigen K40-321 1

General

Antigen Interferon Regulatory Factor 7 (IRF7) Antikörper
Epitop pSer477
(30), (23), (15), (15), (15), (15), (6), (5), (4), (4), (3), (3), (2), (1), (1)
Reaktivität Human
(152), (58), (55), (3), (3), (3), (2), (2), (2), (1), (1)
Wirt Maus
(108), (44)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser IRF7 Antikörper ist konjugiert mit Alexa Fluor 488
(7), (6), (6), (6), (6), (6), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Intracellular Staining (ICS)
(108), (54), (27), (27), (26), (10), (6), (4), (4), (2), (2), (1), (1), (1)
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-IRF7 Antikörper

Target Details IRF7 Anwendungsinformationen Handhabung Referenzen für anti-IRF7 Antikörper (ABIN1177072) Bilder
Marke BD Phosflow™
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human IRF-7 Peptide
Klon K47-671
Isotyp IgG1, kappa
Plasmids, Primers & others

Target Details IRF7

Produktdetails anti-IRF7 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-IRF7 Antikörper (ABIN1177072) Bilder zurück nach oben
Antigen
Andere Bezeichnung IRF-7 (IRF7 Antibody Abstract)
Hintergrund Interferon regulatory factor 7 (IRF-7) is a transcription factor that regulates anti-viral defenses by controlling the induction of type-I interferon (IFN) responses. IRF-7 expression is induced in lymphoid cells by virus infection, as well as by IFN, lipopolysaccharide, and TNF-a. IRF-7 responses are initiated by Toll-like receptors (TLR) or the cytoplasmic protein retinoic acid inducible gene I (RIG-I). Upon TLR activation, it forms cytoplasmic complexes with MyD88, an adaptor in the TLR signaling pathways. The TLR-dependent and RIG-I-dependent pathways activate kinases, such as IKK-e and TBK1, that phosphorylate IRF-7 and induce movement of IRF-7-containing complexes to the nucleus, where it preferentially activates IFN-a promoters. The K47-671 monoclonal antibody recognizes human IRF-7 phosphorylated at serines 477 and 479 (pS477/pS479). Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7. Phosphorylation of IRF-7 in the transfectants requires virus infection or over-expression of a signaling molecule of the RIG-I pathway, such as TBK1. Phosphorylation of endogenous IRF-7 in untransfected cells has not yet been detected. We confirmed that mAb K47-671 does not cross-react with TBK1 by Western blot analysis using the purified antibody.
Pathways TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Autophagie

Anwendungsinformationen

Produktdetails anti-IRF7 Antikörper Target Details IRF7 Handhabung Referenzen für anti-IRF7 Antikörper (ABIN1177072) Bilder zurück nach oben
Probenmenge 20 μL
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-IRF7 Antikörper Target Details IRF7 Anwendungsinformationen Referenzen für anti-IRF7 Antikörper (ABIN1177072) Bilder zurück nach oben
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Referenzen für anti-IRF7 Antikörper (ABIN1177072)

Produktdetails anti-IRF7 Antikörper Target Details IRF7 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Paz, Sun, Nakhaei, Romieu-Mourez, Goubau, Julkunen, Lin, Hiscott: "Induction of IRF-3 and IRF-7 phosphorylation following activation of the RIG-I pathway." in: Cellular and molecular biology (Noisy-le-Grand, France), Vol. 52, Issue 1, pp. 17-28, 2006

Hoshino, Sugiyama, Matsumoto, Tanaka, Saito, Hemmi, Ohara, Akira, Kaisho: "IkappaB kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9." in: Nature, Vol. 440, Issue 7086, pp. 949-53, 2006

Sharma, tenOever, Grandvaux, Zhou, Lin, Hiscott: "Triggering the interferon antiviral response through an IKK-related pathway." in: Science (New York, N.Y.), Vol. 300, Issue 5622, pp. 1148-51, 2003